426 BULLETIN 120, UNITED STATES NATIONAL MUSEUM. 



ojifrinally in formalin have been transferred to alcohol. For the 

 most part, then, Ave will note only the living Aniira which were 

 searched for Opalinidae. 



PISCES. 



Box loops: Leger and Duboscq (1904, b) report th;it all adults at Cavali&re 

 and at Cannes were infected. At Banyuls three-fifths of the young fish, 

 7 centimeters long, were infected. 



URODELA. 



Ambystoma oixicnm: Several living specimens, all uninfected. 



A. maculatum (pioictatum) : o living specimens, all uninfected. 



A. tigrinum: 7 living specimens, all uninfected. Professor Powers, the dis- 

 coverer of Protoopalina mitotica, writes me implying that this species of 

 host, at Lincoln. Nebraska, is frequently infected by this Protoopalina. 



Desmognathus fvscus: 5 living specimens, all uninfected. 



Eurycca ru'bra (Spelerpes rnhrnm) : 6 living specimens, all uninfected. 



Plethodon glutinosns: 3 living specimens, all uninfected. 



Triturvs alpestrift: A single report, Oalli-Valerio (1907), of infection of this 

 species by OpaUnn rannnun. 



T. vulgaris (Triton tacniatvs) : Infection of this si>ecies has several times been 

 reported by European zoologists, but such infection is rare. 



Notophthalmiis viridescens (Diemictylis viridescens) : 17 living specimens, all 

 uninfected. 



Kectrirus maculosus: 2 living specimens, both uninfected. 



We see that three species of Urodela are knoAvn to harbor Opa- 

 linidae. As Triturus is onh' rarely infected, its infection may be but 

 transient and due to the fact that Trifunis feeds upon tadpoles of 

 Anura which regularly carry Opalinidae. However, doubt is cast 

 upon this suggestion by the fact that no Opalinids have been reported 

 from other species of Tritiwus, or from the American Noto2^hthcdmus 

 {Diemictylis). which have similar feeding habits. 



ANURA. 



Family PIPIDAE. 



We have no reports from examination of living members of this family. 



Family DISCOGLOSSIDAE. 



Bomhina bombina {Bombinator igneiis) : Of 63 living specimens examined, 25 

 contained Protoopalina caudata, 15 contained P. intestinalis, 23 contained no 

 Opalinids; that is, about two-thirds were infected. 



Bombina pachypa {Bombinator pachypus) : Of 105 living specimens examined, 

 61 bore Protoopalina caudata; 34 bore P. intestinalis; 1 diseased host bore 

 distorted Protoopnlinae, which may have been P. caudata or P. intestinalis 

 or possibly (?) both species; 8 bore no Opalinids; that is, eleven-twelfths 

 of the individuals were infected. 



The Bombinas are thus seen to be usually infected. 



