THE OPALINID CILIATE IXFUSORIAXS. 427 



Family PELOBATIDAE. 



Scapliiopvs .solitariK.s: Of 4 living speoiniens, 3 curried OiHtlina <)btii;ionoideu. 



Family HYLIDAE. 



Aciis gnjUitx: Of 31 livini;- sitcciuieiis. 7 bore Opuliiia riif/uloidca vuioninu- 

 Virata. 



Chorophilii.s ferianiin: Of 9 living specimens, 3 luul Opalina chorophUi and 6 

 were uninfected. 



Ch. t rise ri at lis: Many living specimens from three localities examined, infec- 

 tion being usual. This statement is made from nieniory. as I have not the 

 notes on the observations. 



Hyla arborea: Of 49 living specimens, 21 were infectetl with Opalina obtrifiona, 

 28 being uninfected. 



H. pickeringii: Many living si)ecimens were examined. The notes of the exami- 

 nations are mislaid. From memory I would say less than half were infected 

 with Opalina obirigonoidea. 



H. versicolor: 2 living adults from Massachusetts were uninfected ; one half 

 grown individual from Michigan was infected with Opalina hylaxena ; several 

 living adults from Ohio were uninfected ; 7 living hirvae from Massachusetts 

 were all infected with Opalina hiiUuena form orbiculata or form parri- 

 nucleata. 



Family BUFONIDAE. 



Bufo bufo (B. vulgaris) : 1 living specimen, examined by Met<alf. bore no 

 Opalinids. Andre (1912, b) reports that of these toads from Switzerland 

 4S.7 per cent of the males and 34. G jier cent of the females were infected with 

 Opalina ranai'Utn or Cepedea denUdiata. 



B. foicleri: Of five living individuals from Massachusetts, 1 bore Opalina- 

 obirigonoidea; of 12 living specimens from Raleigh, North Carolina, 2 bore 

 many Opalina obtrigonoidea. 



B. halophilus: Of numerous living specimens, nearly all were found to be in- 

 fected with Opalina obtrigonoidea maxima. 



B. niaiiniis: Nearly all of about 12 living specimens bore Zclleriella aniil- 

 liensis. 



B. viridis: Of 4 living specimens from Naples, Italy, 1 bore I'rotoopaUna aiu- 

 data; 1 bore Opalina ranarum, and one yielded Cepedea dinfidiata; one was 

 uninfected. 



Family RANIDAE. 



Rana boylei: Numerous living specimens were examined. The notes are mis- 

 laid, but from memory 1 can say that about half were infected with OpaUna 

 virgitloidea. 



R. catesbeiana: ~> living specimens from North Carolina yielded no Opalinids; 

 7 half-grown living specimens from ^lassachusetts bore no Opalinids; 3 

 large living tadpoles from Massachusetts had no Opalinids; 11 full grown 

 living individuals from Maryland bore no Opalinids; several living speci- 

 mens from Ohio were uninfected. No Opalinids have been reported from this 

 species, except that of 15 live specimens from the Hawaiian Islands (in- 

 troduced) 2 bore Cepedea dimidiata hauniiensis. It is said that Anura of 

 several species have been introduced from Asia into Hawaii, and it seems 

 probable that the American bullfrog, also introduced, adopt<^(l its Cepedeas 



