440 BULLETIN 120, UNITED STATES NATIONAL MUSEUM. 



has a single, canal-like, excretory apparatus. [Some species of 

 Opalinidae do have, but not O. ranarum.'] The [morphologically 

 longitudinal] fission is referred to as diagonal. 



Raff (1911) describes and figures the new species Zelleriella 

 {'■'■ O-palzTia'''') hinudeata, from Lininoclysastes dorsalis and L. tas- 

 maniensisy ^nd Protoopalina (^^ Opalina''^) hylarum, from Hyla- 

 aurea. Protoopalina intestinalis [?] is mentioned as present in 

 abundance in Hyla aurea^ H. ewingii, and Limnodynastes dorsalis. 



Stevenson (1911) describes an " Opalina-like form, which I am 

 naming in this paper Protoopalina stevensoni. See p. 50. 



Andre (1912, a). This paper I have been unable to obtain in this 

 country. 



Andre (1912, h) reports that in Bufo Jjufo {''''vulgaris'''') Opalina 

 ranarum and possibly also Cepedeai {'■'■Opalina ") diminiata are 

 present, along with other protozoan parasites, in the intestine, there 

 being no incompatability between the different protozoan parasites. 

 There are different proportions of infection of Bufo hufo in different 

 localities. Of males 48.7 present were found infected, of females 34.6 

 present. 



Hall (1912) mentions " Opalina" [species ?] collected at Eamaley, 

 Boulder County, Colorado, reported by Cockerell in 1911. 



Hartmann (1912) mentions absence of macronuclei in Opalinids; 

 their " anisogamous merogamy (Metcalf, 1909) like that of Ehizo- 

 pods and Sporozoa ; and their " binuclearity [pseudo pleurinuclearity 

 would be more accurate]. 



Metcalf (1912) describes Protoopalina {^'■Opalina'''') mitotica 

 from Amhystonia tigHnum from Lincoln, Nebraska, a new species 

 whose resting nuclei are in a metaphase condition of mitosis. The 

 origin of the binucleated condition of the Ciliata is discussed. \Pro- 

 toopalina mitotica is the first organism, animal or plant, reported 

 whose resting nuclei are in a mid-mitotic condition.] 



Raff (1912) searched the Australian forms Hyla lesueni, Heleio- 

 porus pictus, Pseudophryne seTnimarmorata^ P. hihronii, Crinia sig- 

 nifera^ C. frogatti^ and '■'' HyperolAa'''' [Uperoleial marmorata for 

 Opalinids and describes the following new species ; giving them all, 

 of course, the genus name Opalina; Protoopalina tenuis from Crinia 

 signifera and TJperolela marmorata., P. dorsalis., and P. acuta from 

 TAmnodynastes dorsalis. She reports P. " intestinalis " from TJpero- 

 leia maimiorata. "Abnormal forms " of Zelleriella hinvrcleata ( ? ) , 

 with from two to eight nuclei, are described from a specimen of 

 Liiiinodynastes dorsalis. 



Swarzewsky (1912) refers to Neresheimer's (1907) account of the 

 formation of generative chromidia which give rise to gametic nuclei, 

 also to Metcalf 's (1909) opposed account according to which the 



