REVISION OF PEORIINAE AND ANERASTIINAE 3 



CNHM Chicago Natural History Museum, Chicago, 111. 



CPK Collection of Charles P. Kimball, Sarasota, Fla. 



CU Cornell University, Ithaca, N.Y. 



INHS Illinois State Natural History Survey, Urbaua, 111. 



JCS Collection of the author, Shamokin Dam, Pa. 



JGF Collection of John G. Franclemont, Ithaca, N.Y. 



KSU Kansas State University, ISIanhattan, Kans. 



LACM Los Angeles County Museum, Los Angeles, Calif. 



MOG Collection of IMurray O. Glenn, Henry, HI. 



UCB University of California, Berkeley, Calif. 



UK University of Kansas, Lawrence, Kans. 



UM University of Minnesota, St. Paul, Minn. 



USNM U.S. National Museum, Washington, D.C. 



Techniques. — Preparatoiy to examining the genitalia the abdomen 

 was broken away from the thorax and macerated in cold 10 percent 

 potassimn hydroxide (KOH) solution for from 4 to 8 hours, then 

 dissected in 50 percent alcohol. The abdomen was carefully torn open 

 with jewelers' forceps along the entire right plem-al area, enabling the 

 cuticle to be mounted flat with the outer surface upward. The genitalia 

 were separated by carefully tearing along the membrane between 

 segments eight and nine. After the removal of loose scales and KOH, 

 the male genitalia were usually stained with mercmochrome (0.3 

 percent aqueous solution), or occasionally with chlorazol black or 

 acid fuchsin to improve rendition of various structures. The aedeagus 

 was removed, and the valves were spread and held open by a small 

 rectangular section of microscope slide placed over the genitaha. The 

 genitalia were held in this position in 95 percent alcohol until hardened, 

 then transferred successively to clove oU and xylene for about 15 

 minutes each, prior to mounting in Canada balsam on a microscope 

 slide. 



Female genitalia were put through the same series of chemicals as 

 those of the males, but were usually stained with chlorazol black or 

 mercm-ochrome. 



In order to allow observation from any desired angle the genitalia 

 of at least one specimen of each sex of each species were transferred 

 from xylene into small (5 x 10 mm) genitalia vials, along with a drop 

 of Cargille's type A microscope immersion oU; the males were left 

 unspread. These specimens were eventually washed in xylene and 

 moimted in balsam; before mounting they proved very useful in 

 preparing descriptions because it was possible to study the relation- 

 ships of the various structures. 



Attempts to spread the male genitalia of the Peoriinae in the 

 manner described above caused considerable distortion; thus the 

 method had to be modified. If one will visualize the external male 

 genitaha as the aedeagus and a surrounding cyhnder, and then the 

 cylinder as severed lengthwise, the genitalia can be "unrolled" and 



