L6 



THE A(TIX(>.MV('KTi:S, Vol. II 



Figure 4. .1. israelii, grown : 

 Institute of Pathology). 



Forces 



branching, with prevailing development of 

 one branch. 



Glucose agar: Discoid, moist, and brilliant 

 colonies; slightly elevated in the central pari 

 with nearly regular margins. 



Gelatin: Xo liquefaction. 



Glucose broth: Slimy sediment and some- 

 times a slight turbidity. The medium be- 

 comes clear at the end of 8 to 10 days. 



Carbon sources: Acid but no gas from glu- 

 cose, maltose, fructose, lactose, sucrose, and 

 iiiulin; very little or no acid from mannitol. 



Starch: Not attacked. 



Sucrose: Not inverted. 



Nitrate reduction: Negative. 



H 2 S: Formed. 



Indol: Slight quantity produced. 



Fats: Slightly attacked. 



( Hive oil: Not attacked. 



Optimum temperature: 37°C. 



( >xygen demand: Facultatively anaerobic. 



Remarks: Vitality weak. Deep cultures in 

 semisolid media die if held for longer than 8 

 to 10 days at 37°C, or for longer than 30 

 minutes at 60°C. Exposure for longer than 



a few minutes in dilute mineral acids kills 

 the organism. The organism can be kept 

 alive for 2 to 3 months if cultures are kept 

 in an ice chest, in a dried state, or under 

 vacuum. 



Habitat: Lachrymal concretions and hu- 

 man actinomycotic lesions. 



5. Actinomyces hvidhanseni (Hvid-IIan- 

 sen, 1951) nov. comb. (Hvid-Hansen, X. 

 Acta Pathol. Microbiol. Scand. 29: 335-338, 

 1951). 



Synonym: Actinomyces Israeli Hvid-Han- 

 sen. 



Morphology: Gram-positive, nonacid-fast , 

 nonmotile. Polymorphic, bent, and often 

 branched rods. Obligately anaerobic. 



Meat liver agar: Colonies circular or ir- 

 regular, often in the form of bodies bounded 

 by four concave surfaces meeting in four 

 acute vertices, of highly varying size and of 

 a pale pink color. Surface colonies circular, 

 convex, grayish-white or white; transparent 

 S-colonies of a butyrous, viscous, but not 

 mucous consistency. 



Meat liver broth: Diffuse growth at first, 



