32 



THE ACTINOMYCETES, Vol. II 



3. Classification of Nocardia, According 

 to the system of Waksman and 

 Henrici* 



A. Partially acid-fast organisms with strongly re- 

 fractive cells; nonproteolytic and generally 

 nondiastatic; capable of utilizing paraffin. 

 I. Initial mycelium fully developed, well 

 branching, dividing into rods and generally 

 into cocci. 



1. Substrate growth soft, without macro- 

 scopically visible aerial mycelium. 



a. Substrate mycelium yellow, orange, 

 or red. 



a 1 . Pathogenic. 



a 2 . Substrate mycelium white, 

 buff, or pale yellow. 



18. Nocardia farcinica 

 b 2 . Substrate mycelium yellow to 

 red. 



6. Nocardia asteroides 

 b 1 . Not pathogenic. 



a 2 . Paraffin decomposed. 



42. Nocardia polychronio- 

 genes 



b 2 . Cellulose decomposed. 



13. Nocardia cellulans 



b. Substrate mycelium white to pink. 

 a 1 . Gelatin not liquefied. 



a 2 . Growth on nutrient agar 

 opaque, cream-colored. 

 38. Nocardia opaca 

 b 2 . Growth on nutrient agar pink. 



10. Nocardia calcarea 



a 3 . Aerial mycelium on milk 

 white. 



31. Nocardia leishmanii 

 b 3 . Pellicle on milk pink. 



11. Nocardia caprae 



c 3 . Pellicle on milk yellow. 

 9. Nocardia brasiliensis 

 d 3 . Causing galls on blueberry 

 plants. 



53. Nocardia vaccinii 

 b 1 . Gelatin liquefied. 



43. Nocardia pulmonalis 



2. Substrate mycelium hard, yellow. 



a. Aerial mycelium white; hyphae di- 

 vides into chains of acid-fast cocci. 

 40. Nocardia paraffinae 



* This system was used, with certain minor 

 omissions and additions, in the last edition of 

 Bergey's Manual. 



b. Aerial mycelium not produced on or- 

 ganic media. 



41. Nocardia petroleophila 



3. Substrate growth cream colored, later 

 becoming yellow. 



51. Nocardia serophila 



4. Substrate grow T th hard, orange-yellow. 



58. Nocardia variabilis 



II. Initial mycelium very short, rapidly divid- 

 ing into rods and cocci. 



1. Growth pink. 



a. No cystites (swollen cells) formed. 

 a 1 . No indigotin from indole. 



16. Nocardia corallina 

 b 1 . Indigotin from indole. 



26. Nocardia globerula 



b. Cystites formed. 



49. Nocardia salmonicolor 



2. Growth coral -red. 



47. Nocardia rubropertincta 



3. Growth white, tan, or pink. 



a. No aerial mycelium. 

 a 1 . Growth tan. 



15. Nocardia coeliaca 

 b 1 . Growth white. 



28. Nocardia intracellular is 



b. Aerial mycelium produced. 



a 1 . Growth frequently pinkish. 



53. Nocardia transvalensis 

 b 1 . Growth never pink. 



50. Nocardia sebivorans 



4. Produces no pigment, no growth on 

 potato, coagulates milk. 



19. Nocardia fastidiosa 



B. Nonacid-fast organisms with weakly refrac- 

 tive cells; no distinct formation of cocci; di- 

 astatic. 

 I. Nonproteolytic, although some give gela- 

 tin liquefaction. 



1. Growth on agar pale cream. 



a. Gelatin not liquefied; starch hy- 



drolyzed. 



35. Nocardia mcsentcrica 

 1). Gelatin liquefied; starch not hy- 



drolyzed. 



48. Nocardia rugosa 



2. ( irowth on agar whitish. 



4. Nocardia albicans 



3. Growth on agar yellow. 



20. Nocardia flava 



4. Growth on agar green. 



59. Nocardia viridis 



5. Growth on agar yellow-green. 



14. Nocardia citrea 



