THE GENUS NOCARDIA 



37 



Remarks: Another closely related culture 

 lias been described as Proaetinomyces flavus 



(sec .V. marina I. 



8. Nocardia blackwellii (Erikson, L935) 

 W'aksniaii and Henrici, L948 (Erikson, 1). 

 Med. Research Council Spec. Repl . Ser. 203: 

 32 33, L935). 



Morphology: Growth consisting of short, 

 rod-like filaments, growing out into longer 

 sparsely branching hyphae. Aerial mycelium 

 short, straight; frequently Large, round or 

 ovoid cells are interposed in the irregularly 

 segmented chains of cells. 



Glycerol nitrate agar: Growth extensive, 

 granular, irregular, thin, pinkish. 



Nutrient agar: Growth confluent, wrin- 

 kled, with small, round, pinkish, discrete 

 colonies at margin. 



Glucose nutrient agar: Growth abundant, 

 pale pink, in form of small conical colonies, 

 piled up, convoluted. 



Potato agar: Colonies small, round, color- 

 less. Aerial mycelium white. Later, colonies 

 dull pink, submerged margins; few aerial 

 spikes, moderate aerial mycelium at top of 

 slant. 



Gelatin: Colonies few, colorless, minute, 

 along line of inoculation. Later, abundant, 

 colorless colonies to 10 mm below surface; 

 larger pink-yellow surface colonies with 

 white aerial mycelium. No liquefaction. 



Milk: Surface pellicle heavy, convoluted, 

 bright yellow. No coagulation; no peptoniza- 

 tion. Spalla states that milk is coagulated. 



( Jarbon utilization: See Table 4. 



Source: Hock joint of foal. 



Type culture: ATCC 6846; NCTC 630. 



!). Nocardia brasiliensis I Lindenberg, L909) 

 Castellani and Chalmers (mend. Gordon and 

 Mihm, 1959 (Lindenberg, A. Arch. Parasitol. 

 13: 2(15-282, 1909; Castellani, A. and Chal- 

 mers, A. .1. Manual of Tropical Medicine, 2d 

 Ed. William Wood & Co., 1913, p. 816; 

 Cordon, R. E. and Mihm, .1. M. .1. Gen. 

 Microbiol. 20: 121), L959). 



Synonyms: Gonzalez Ochoa I 1945, 1953), 

 Gonzalez Ochoa and Sandoval (1956), and 

 Gordon and Mihm (1958) consider A', bra- 

 siliensis a- the proper name for ihi- organ- 

 ism. .1. mexicanus Boyd and Crutchfield, 

 A', pretoriana Pijper and Pullinger, and A'. 

 transvalensis Pijper and Pullinger, are con- 

 sidered as synonyms. 



Morphology: Angularly branched fila- 

 ments, bearing a few short straight aerial 

 hyphae; later, growth becomes spreading 

 and extensive. Aerial hyphae long and 

 branching to short and gnarled; divide to 

 form oval and cylindrical spores. Acid-fast- 

 ness variable, from 100 per cent to none. 



Glycerol nitrate agar: Growth in form of 

 piled up pink mass. Aerial mycelium very 

 scant , white, at margin. 



Glucose nutrient agar: Colonies pale buff, 

 umbilicated and piled up. 



Yeast-glucose agar: Highly mutable. 

 Growth yellow to yellowish-orange, finely 

 wrinkled. Some strains produce no aerial 

 hyphae; other strains form mat of whitish 

 aerial hyphae. A few strains form amber to 

 brown soluble pigment. 



Potato: Colonies small, raised, pale pink; 

 plug and liquid discolored. Later, growth 

 dull buff, dry and convoluted at base, round 

 and zonate at top of slant. Aerial mycelium 

 white. 



Gelatin: A few colorless flak*-. No lique- 

 faction 



Milk: Surface growth yellowish. Pale pink 

 growth up the wall of the tube. Solid COagU- 

 lum in 1 month; later, partly digested. 



Egg medium: Colonies few, round, color- 

 less in :! days. Later, irregular, raised pink 

 mass, warted appearance; moderate degree of 

 liquefaction. 



Serum agar: Growth raised, convoluted, 

 slighlly pinkish. 



Source: A case of mycetoma of the che>t 

 wall in a South African native. 



Pathogenicity: To guinea pigs and humans. 



Remarks: According to Cordon and 



