THE GENUS VOCARDIA 



::«.) 



becoming dull pink, 



while aerial myceliun 



Potato: Growth al 



;ular, with -cant raised, pale pink, confluent growth. Aerial 



mycelium white. Plug discolored. 

 nt. Aerial myce- Gelatin: A few colorless flakes. No lique- 



li. 



pale pink. Growth becomes membra- faction. 



nous, considerably buckled. 



Gelatin: Growth extensive, dull, with 

 small raised patches of pink aerial mycelium; 

 later, ribbon-like, depressed. No liquefac- 

 tion. 



Milk: Surface pellicle red. Solid coagulum; 

 no peptoni/.at ion. 



Starch: No hydrolysis. 



Source: Lesions in goats. 



Pathogenicity: To rabbits, guinea pigs, 

 and mice. 



Milk: Surface growth colorless. Aerial 

 mycelium white. Coagulation positive. 



Nutrient broth: Surface pellicle cream- 

 colored, wrinkled, extending up wall and 

 breaking easily; moderate bottom growth, 

 flaky. Medium discolored. 



Source: Infected guinea pigs from Su- 

 matra. 



Remarks: Schneidau and Shaffer (1957) 



report that the organism is not acid-fast, 



grows at 46°C, utilizes paraffin, liquefies 



Remarks: According to Gordon and Mihm gelatin, hydrolyzes casein, liquefies starch, 



(1959) A', caprat is a synonym of A', aste- and shows positive hemolysis. 



routes; according to Schneidau and Shaffer 1; . Nocardia cellulans Metcalfe and 



I L957), however, the organism does not uti- 1WvM ,,,- 7 ( Metcalfej <;. and Browllj M 



li/e paraffin and does noi hemolyze blood, K ,, (>11 Microbiol. 17: 569 570. L957). 



as shown on p. (IS, Volume I. Morphology: Gram-positive and partially 



Type culture: IMRI 783. acid-fast. Branching aseptate filaments. 30 



12. Nocardia caviae Snijders, 1924 (Snij- i<> 40 ^ in length, often with swellings at in- 



ders, Geneesk. Tijdschr. Ned. Indie 64: 47. tervals; shorter filaments are less than 7.0 m 



7."), P.I24). in length. Fragmentation commences aboul 



Morphology: Growth consists of initial the fourth day, the number of shorl rods 



segmented hyphae, producing elements of (1.5 to 2.0 by 1.0 m) increasing rapidly. 



approximately even thickness, arranged in Hyaline spore-like structures are produced 



from the seventh day as slight terminal 

 swellings on the filaments. After 28 days, the 

 colonies consist of very short rods, cocci and 

 spore-like cells. 



Agar media: Colonies raised, soft, without 

 aerial mycelium; cream-colored on most 

 media hut characteristically bright yellow on 

 yeasl ex1 ract-peptone agar. 



Glucose agar: Filaments fragment less 

 rapidly and are occasionally found after 28 

 days. Numerous Y-forms .-ire found in older 



cultures. 



Cellulose tubes: After ii days there are 

 long (20 to 30 n) branched and unb 



angular apposition; later, forms long, pro- 

 fusely ramifying threads with strongly re- 

 tractile protoplasm. Aerial mycelium straight 

 and branching, the sporophores forming oc- 

 casional coiled tips, divided into cylindrical 



-poll'-. 



Glucose agar: Growth piled up. convo- 

 luted, cream-colored to pale pink. Aerial 

 mycelium white. 



Glycerol agar: Growth scanty. 



Potato agar: Growth spreading, colorless. 

 Aerial mycelium dense white. 



Egg medium: Growth heavily corrugated. 

 pale pink, with submerged margin. Aerial filaments, many with terminal swelling 

 mycelium dense while. After 3 weeks, color- Fragmentation is rapid and shorl rods and 

 less transpired drops. cocci predominate during the stage of active 



Potato: Colonies -mall, colorless. Aerial cellulose decomposition, old cultures are 

 mycelium white, powdery. Later, abundant, composed almos! entirely of cocci. 



