THE GENUS NOC IRD1 I 



II 



Nutrient agar: Colonies smooth, pink, 

 shining; border lighter, edge filamentous or 

 with arborescenl projections. As the colony 

 grows, the cells in the interior break up into 



short rods and cocci which eventually form 

 the mass of the colony. Cells on the outside 

 remain filamentous, giving the colony a 

 burr-like appearance, and often forming long 

 arborescenl processes. No soluble pigment. 



Potato-glycerol agar: Growth filiform, 

 raised, dry, wrinkled, yellowish-brown to 

 coral red. 



Gelatin: Surface colonies round, convex, 

 smooth, pink, shining, edge filamentous; 

 deep colonies, burrs. No liquefaction. 



Milk: Reddish pellicle; milk becomes alka- 

 line. 



Nutrient broth: Usually turbid. Pink 

 scum. 



Paraffin and phenol: Utilized. 



Nitrate reduction: Positive. 



Starch: Not decomposed. 



Sucrose: Not inverted. 



Egg medium: Filiform, raised, dry, 

 wrinkled, orange. 



Temperature: Optimum 22-25°C. 



Habitat : Soil. 



Remarks: Some strains produce acid from 

 glycerol and glucose. No acid or gas from 

 sucrose, maltose, or lactose. Phenol and nt- 

 cresol are utilized. Some strains utilize 

 naphthalene. Krassilnikov (1949) reports for 

 his strains, good growth in high salt concen- 

 trations. Schneidau and Shaffer I 1957 I reporl 

 positive acid-fastness, positive hemolysis 

 and urease formal ion. 



17. Nocardia dicksonii (Erikson, 1935) 

 Waksman (Erikson, I). Med. Research 

 Council Spec. Rept. Ser. 17: 203, 1935). 



Morphology: Growth consists of long fila- 

 ments, sometimes wavy. Aerial mycelium 

 straight . Spores cylindrical. 



Glycerol nitrate agar: Growth granular 



and wrinkled, cream-colored. Medium deeply 

 discolored. 



Glucose-asparagine agar: Growth wrin- 

 kled, colorless. 



Potato agar: ( '.row th abundant , colorless. 

 Egg medium: Growth yellowish-brown. 

 Starch agar: Strong hydrolysis. 

 Gelatin: ( '.row th smooth, cream-colored on 

 surface. Liquefaction limited. 

 Milk: Coagulated, peptonized. 

 I labitat : I nknown. 



18. Nocardia fun-mica (Nocard, 1888) 

 Trevisan and De Tom, L889 'Nocard. M. 

 E. Ann. inst. Pasteur, 2: 293, 1888; Trevi- 

 san, V., I. generi e le specie delle Batleri- 

 acee, Milan, 1889, p. ( .»). 



Morphology: Growth yellow, of doughy 

 consistency. Markedly acid-fast. 



Nutrient agar: Colonies yellowish- white, 

 irregular, refractive; mycelium filamentous. 



Potato: ( Irowth abundant , dull, crumpled, 

 whitish-yellow. 



Gelatin: Colonies small, circular, trans- 

 parent, glistening. No liquefaction. 



Milk: No coagulation; no peptonization. 



Starch: No hydrolysis. 



Nutrient broth: Clear, with granular sedi- 

 ment, often with gray pellicle. 



Nitrate reduction: Negative. 



Temperature: Optimum .'>7°0. 



Pathogenicity: Pathogenic to certain 

 domestic animals and guinea pigs. 



Source: Cases of cattle farcy. 



Type culture: IMP!' .'CIS. 



19. 

 L. S. 



M< 

 on al 

 striki 



Nocardia fastidiosa Suter, 1951 (Suter, 

 Mycologia 13: 658 676, 1951 I. 

 irphology: The organism- were similar 

 1 media studied, showing in general a 

 ng pleomorphism with coccoid, bacil- 

 and filamentous forms. Many of these 

 clubbed; some bore a striking resem- 

 •»• to spermatozoa. Other- were thick at 



•nd and tapered down to filamentous 

 Filamentous forms were up to 25 m in 

 h and. not considering clubs or swollen 

 ons, measured 0.2 to 1.2 ^ in diameter. 

 verage being about 0.8 to 1 (t. Stained 



