THE GENUS VOC \.RD1 I 



i:; 



diameter. Some strains form chlamydo- 

 spores. Numerous inflated cells of the bulbi- 

 form or fusiform type. ( Jell mull iplical ion by 

 fission, cross wall formation, rarely by bud- 

 ding. \'oi acid-fasl . 



Synthetic agar: Colonics brighl yellow or 

 golden. 



Nutrient agar: Growth dirty, lustrous, or 

 rough and folded, of a dough-like consist- 

 ency, yellow to straw-colored. No soluble 

 pigment. 



( ielat in : No liquefad ion. 



Milk: Xo coagulation and no peptoniza- 

 tion. 



Starch hydrolysis: Slight . 



Sucrose: Weak inversion. 



Cellulose: No growth. 



Paraffin and wax: Xo growth. 



Fat : Weak growth. 



Habitat: Soil. 



21. Nocardia flavescens (Jensen, L931) 

 Waksman and Henrici, L948 (Jensen, H. 

 Proc. Linnean Soc. X. S. Wales 56: 301, 

 L931). 



Morphology: Substrate growth forms long, 

 luanched, nonseptate hyphae, 0.4 to 0.6 fx. 

 On nutrient agar and potato, septa are 

 formed, mycelium fragmenting, partly re- 

 sembling highly branched mycobacteria. 

 Aerial mycelium consists of fairly long 

 hyphae of the same thickness as the vegeta- 

 tive hyphae, not very much branched, with- 

 out spirals, often clinging together in wisps; 

 hyphae break up into fragments of variable 

 lengths, from 1.2 to 1 .5 up to 10 to 13 m, 

 showing an irregular, granulated staining. 

 Xot acid-fast. 



Nutrient agar: Substrate growth raised 

 and much wrinkled, first dirty cream-col- 

 ored, later dark yellowish-gray, of a soft, 

 moist, curd-like consistency. Aerial myce- 

 lium absent. Soluble pigment absent. 



Glucose agar: Substrate growth super- 

 ficial, wrinkled, honey-yellow, of a hard 

 and cartilaginous consistency. Aerial myce- 



lium thin, smooth, white. Soluble pigment 

 yellow. 



Potato: Substrate growth much raised 

 and wrinkled, first cream-colored, later 

 yellowish-brown, soft and smeary. Xo aerial 

 mycelium. Xo soluble pigment . 



( ielatin: Liquefad ion slow. 



Milk: Coagulation; slow peptonization 

 with acid reaction. 



Starch: i Iydrolyzed. 



Cellulose: Xo growth. 



Paraffin: Xo growth. 



Sucrose: Inverted. 



Glucose broth: Rather scant growth. 

 Granulated, yellowish sediment ; no surface 

 growth. Broth clear. Xo pigment. Xo acidity. 



Nitrate: Slight or no reduction. 



Source: Soil. 



22. Nocardia fordii (Erikson, L935) Waks- 

 man (Erikson, D. Med. Research Council 

 Spec. Rept. Ser. 203: 15, 1935). 



Morphology: Substrate growth consists of 

 filaments of medium length. Aerial myce- 

 lium short, straight, sparse. Small oval 

 spores on potato agar and starch agar. 



Glycerol nitrate agar: Growth thin, ex- 

 tensive, golden brown, convoluted. 



Xutrient agar: Colonies small, creamy 

 to golden, ring-shaped; later, heaped-up 

 patches, becoming golden brown and con- 

 voluted. 



Egg medium: Colonies minute, cream- 

 colored, elevated, becoming golden brown, 

 raised. 



Potato: ( rrowth yellowish in thin terminal 

 portion, tending to be piled up. Aerial myce- 

 lium scant, white, at top of slant. Later, 

 growth abundant, golden brown, confluent, 

 partly honeycombed, partly piled up. 



Gelatin: No visible growth, slighl soften- 

 ing of gelatin; later partial liquefaction. 



Milk: Surface ring brownish. Coagulation 

 positive. 



Starch: Xot hydrolyzed. 



Source: Human spleen in a case of acho- 

 luric jaundice. 



