THE GENUS N0CARD1 



15 



lowish, irregularly wrinkled, clear hemolytic 



zone. 



Egg medium: Colonies small, round, 

 smooth, colorless, with conically elevated 



cciilcrs. 



Potato: No growth. 



Gelatin: Dull white flakes sinking as me- 

 dium liquefies. Liquefaction rapid. 



Milk: Coagulation positive; peptoniza- 

 tion limited. 



Starch: Not hydrolyzed. 



Source 1 : Human spleen in a case of acholuric 

 jaundice. Injected into a monkey and reiso- 

 lated. 



Type culture- ATCC 6852. 



26. Nocardia globerula (Cray, 1928) Waks- 

 maii and Ilenrici, 1948 (Gray, P. Proc. Roy. 

 Soc. (London) B 102: 265, 1928). 



Morphology: Growth orange to orange- 

 buff. It consists of curved rods and fila- 

 ments, 1 by 2 to 9 ju, with many coccoid cells, 

 especially in old cultures. Rods and filaments 

 frequently irregularly swollen. Not acid-fast. 

 Capsules may be present. 



Nutrient agar: Surface colonies irregu- 

 larly round, 3 to 5 mm in diameter, convex, 

 white, smooth, shining; edge undulate, erose. 

 Deep colonies, lens-shaped. 



Gelatin: Surface colonies irregularly 

 round, 1 to 2 mm in diameter, convex, light 

 buff, smooth, shining. Stab: nailhead, irreg- 

 ularly round, convex, pinkish-white, smooth, 

 shining. 



Potato-glycerol agar: Growth filiform, 

 moisl , smooth, pale pink. 



Milk: Alkaline. 



Nutrient and peptone broth: Turbid with 

 viscous suspension. 



Nitrate reduction: None. 



Egg medium: Growth spreading, raised, 

 moist , orange-colored. 



Indole agar: Blue crystals of indigotin 

 formed. 



Temperature: Optimum 25-28°C. 



Phenol: Utilized. 



Source: Soil. 



Remarks: This organism resembles most 

 closely A', corallina. It is distinguished by 

 producing a more watery type of surface 

 growth, more nearly entire deep colonic-, 

 and more particularly by the production of 

 indigotin from indole. No acid from glucose, 

 lactose, maltose, sucrose, or glycerol. 



Type culture: ATCC 13,130. 



27. Nocardia hortonensis (Erikson, 1935) 

 Waksman (Krikson, D. Med. Research 

 Council Spec. Kept. Ser. 203: 22, 1935). 



Morphology: Substrate growth made up 

 of very slowly developing unicellular myce- 

 lium, composed of long slender straight 

 branching filaments. Aerial mycelium very 

 sparse, forming straight hyphae only once 

 on potato. Not acid-fast . 



Glycerol nitrate agar: Colonies coiled, 

 colorless, lustrous patches, isolated, with 

 central depression. 



Nutrient agar: Growth very slow, as few 

 smooth, cream-colored, coiled colonies. 



Glucose nutrient agar: Growth as coiled 

 and heaped up cream-colored translucent 

 masses. 



Potato agar: Colonies colorless, blister; 

 later dull green heaped and coiled mass. 

 Medium becomes slightly discolored. 



Potato: Colonies abundant, colorless, 

 umbilicated, round, some coiled in raised 

 masses; later, liberal olive-green growth. 

 Aerial mycelium dense, velvety gray-green 

 at top of slant . 



Gelatin: Colonies round, cream-colored on 

 surface and a few millimeters below. No 

 liquefaction. 



Milk: Surface growth green; peptoniza- 

 tion positive. Color at first purple, later 

 brown. 



Source: From pus containing typical 

 actinomycetic granules from parotid abscess. 



28. Nocardia intracellularis Cutting and 

 McCabe, MM!) (Cutting, J. T. and McCabe, 

 A. B. Am. .1. Pathol. 23: I 17, L949). 



Morphology: Filaments branched, becom- 

 ing fragmented, composed of bacillary ele- 



