Hi 



THE ACTINOMYCETES, Vol. II 



ments in scries, 0.2 to 0.45 m in width. Liquid 

 cultures give branched colonies. The hyphae 

 do not form club-shaped tips, and lack 

 chlamydospores. Not discolored when 

 stained with fuchsin and treated with acid 

 alcohol. 



Agar media: Colonies circular, raised, 

 wet-shining, smooth, and nonmucoid. 



Potato agar: No growth. 



Gelatin: Growth poor. No liquefaction. 



Glycerol broth: White, mucoid masses 

 formed at bottom of lube. Medium remains 

 clear. 



Milk: Acid after 20 to 30 days. 



Starch: Not changed. 



Tyrosinase reaction: Absent. 



Cellulose: Not decomposed. 



Nitrate reduction: None. 



Oxygen requirement: Does not, develop 

 in the absence of oxygen, but grows in an 

 atmosphere having 10 per cent C0 2 . 



Paraffin: Used as the only source of car- 

 bon. 



Temperature: Grows at 37.5°C and tol- 

 erates well temperatures up to 40°C. 



Habitat: Observed in granuloma of in- 

 fected lymph nodes and in the feces of a 

 living patient whose death it ultimately 

 caused. Observed also at autopsy in widely 

 disseminated granulomatous lesions which 

 it produced. 



Type culture: ATCC 13,209. 



2'.). Nocardia ivorensis Combes, Kauff- 

 mann and Vazart, 1957 (Combes, R., Kauff- 

 mann, J., and Vazart, B. Compt. rend. 224: 

 821-824, 1582-1587, 1957). 



Agar media: Substrate growth character- 

 ized by the black coloration of its coccoid 

 bodies, by their elongation, by their resist- 

 ance io dryness and to heat, by their cellulo- 

 lytic properties, and by their production of 

 an orange pigment on different media. Col- 

 onies at first whitish, centers becoming light 

 orange to brown, later black. Black circles 

 formed successively around the central circle 

 and finally becoming confluent. Later, sur- 



face of colony is uniformly black, shiny, and 

 waxy; at the periphery, grayish, radiant 

 outgrowths develop in the agar, forming a 

 more or less regular fringe; on the surface of 

 this fringe brown, rapidly darkening, con- 

 centric zones appear. 



Milk: Reddish-orange surface film, and 

 isolated colonies adhering to the walls of 

 the tube. 



Potato: Growth orange, darkening in 

 places, with the appearance of coccoid forms. 



Gelatin: Growth scant, slow; later the 

 culture is orange in color. Liquefaction 

 positive. 



Cellulose (filter paper or washed cotton), 

 moistened with the synthetic medium: Light 

 brown colonies appear in 2 days and turn 

 dark at 6 days, being entirely composed of 

 coccoid forms; later, colonies are entirely 

 black. Cellulose progressively disintegrates. 



Paraffin : White colonies appear at 5 days. 

 They remain small and rapidly form coccoid 

 elements. 



Nitrate reduction: Positive. 



Remarks: Three isolated cultures differed 

 from each other mainly in the rapidity with 

 which they formed coccoid elements. Or- 

 ganism closely related to N. nigra. 



Habitat: Colony of termites on ivory 

 coasi of Africa. 



30. Nocardia kuroishi Uesaka, 1952 (Ue- 

 saka, I. J. Antibiotics (Japan) 5: 75-79, 

 1952). 



Morphology: Aerial mycelium abundant. 

 Sporophores slightly curved at first, later 

 turning around each other. Acid-fast. 



Glycerol nitrate agar: Growth thin, pale 

 yellow. Aerial mycelium punctiform, white. 

 Soluble pigment yellow. 



Nutrient agar: Colonies wrinkled, grayish- 

 yellow. No aerial mycelium. Soluble pigment 

 faint grayish-brown. 



Glucose nutrienl agar: Growth abundant, 

 at first yellowish-brown, then reddish-brown. 

 Aerial mycelium scant, white at margin of 



