THE GENUS NOCARDIA 



17 



colonics. Soluble pigmenl red to wine- 

 colored. 



Potato: Growth moderate, at first red or 

 brownish red, later dark brown. Aerial myce- 

 lium grayish white. Soluble pigmenl dark 

 brown. 



Glucose broth: Red colonies forming 

 pellicle. Abundant, flocculenl sediment. 

 Soluble pigmenl dark brown. 



Gelatin: Growth yellowish-brown, sinking 

 into medium. No aerial mycelium. No lique- 

 faction. Soluble pigmenl yellowish-brown. 



Milk: No coagulation. Slow peptonization. 

 Brown pigmenl . 



Starch: Hydrolyzed. 



Carbon source: Lactose well utilized. 



Nitrate reduction: None. 



Antagonistic properties: Produces an 

 antibiotic neonocardin, active againsl vari- 

 ous bacteria. 



Source: Soil. 



31. Nocardia leishmanii Chalmers and 

 Christopherson, 1916 (Bin, ('. and Irish- 

 man, W. B. J. Byg. 2: 120, L902; Chalmers, 



A. and Christopherson, I. Ann. Trop. Med. 

 Parasitol. 10: 255, 1916). 



Morphology: Initial cells frequently swol- 

 len, large and irregular, aggregated in shorl 

 chains and then branching out into regular 

 narrow filaments; later entire colonics as- 

 teroid in appearance, very fine and close 

 angular branching, with aerial hyphae situ- 

 ated singly. Whitish-pink aerial mycelium 

 generally abundant with irregularly cylin- 

 drical conidia. Acid-fast. 



Glucose nutrient agar: Colonies rounded, 

 elevated, red, with paler frosting of sparse 

 aerial mycelium. No soluble pigment. 



Glycerol agar: Colonies small, round, pink, 

 tending to be umbilicated and piled up. 

 Aerial spikes stiff, white. 



Potato agar: Colonies minute, colorless, 

 round. Aerial mycelium white, in patches. 



Egg medium: Growth colorless, confluent, 

 studded with little wart-like projections 



hearing stiff aerial spikes; later pinkish. 

 Aerial mycelium white. Medium discolored. 



Gelatin: Colonies small, pink. No lique- 

 faction. 



Milk: Sin-face growth; aerial mycelium 

 white turning pink. Coagulum solid, later 

 partly peptonized. 



Pathogenicity: To rabbits, rat-, and 

 guinea pig-. 



Source: Fatal case of lung disease and 

 pericardii is in man. 



Remarks: According to Gonzalez Ochoa 

 and Sandoval (1956), A', leishmanii is a 

 synonym of A'. asU roidi zs. 



32. Nocardia listen (Erikson, L935) Waks- 

 mann (Erikson, I). Med. Research Council 

 Spec. Rept. Ser. 203: 23 24, L935). 



Morphology: Sporophores short and 

 straight . Spores oval. 



Glycerol nitrate agar: Growth abundant, 

 moist, cream-colored. Aerial mycelium pow- 

 dery, white, with exuded drops. 



Calcium malate agar: Growth poor, in 

 form of a biscuit-colored membrane. 



Nutrient agar: Growth smooth, moist, 

 cream-colored, margin depressed, center 

 elevated. 



Glucose nutrient agar: Growth cream- 

 colored, glistening. 



Potato agar: Growth extensive, colorless, 

 warted surface. Dirty pink coloration after 

 2 weeks. Scant white aerial mycelium after 

 1 months. 



Potato: ( irowth abundant . dull, brownish, 

 wrinkled. Aerial mycelium white. 



Gelatin: Surface colonies round, white; 

 after 45 days, confluent -kin. Liquefaction 

 -light. 



Blood agar: Colonic- small, round, cream- 

 colored, with smooth, translucenl surface. 



No hemolysis. 



Serum agar: Colonies -mall, irregular, 

 moist, cream-colored, tending to be heaped 

 up; later somewhat transparent. 



Milk: Coagulated. No change in reaction. 



Source: From human material. 



