IS 



THE ACTINOMYCETES, Vol. II 



33. Nocardia lutea Christopherson and 

 Archibald, 1918 (Christopherson, J. B. and 

 Archibald, R. G. Lancet 2:847, 1918).* 



Morphology: Growth consists of irregular, 

 spreading, polymorphous colonies, compris- 

 ing swollen and segmented cells of all shapes 

 and sizes with markedly granular contents. 

 Later cells more monomorphous, the fila- 

 ments being arranged in angular apposition. 



Glycerol nitrate agar: Growth in form of 

 yellowish-pink, wrinkled membrane. 



Nutrient agar: Growth abundant, co- 

 herent, moist, pink, membranous with round 

 discrete colonies at margin. 



Glucose nutrient agar: Growth scant, 

 reddish, smeary. 



Potato agar: Small filamentous colonies 

 are formed; irregular angular branching. 

 Aerial hyphae few, isolated, short, straight. 



Potato: Growth carrot-red, moist, thick, 

 granular in bands, partly raised and with 

 discrete round colonies. Aerial mycelium 

 sparse, colorless, very thin at top of slant. 



Gelatin: Growth pale pink, wrinkled on 

 wall of tube. Colorless punctiform and stel- 

 late colonies in medium. No liquefaction. 



Milk: Growth orange-red on surface and 

 at bottom. 



Egg medium: Growth poor, dull pink. 



Source: Actinomycosis of the lachrymal 

 gland. 



Remarks: According to Erikson, various 

 saprophytes, such as N. rubra and N. poly- 

 chromogenes, are closely related. 



34. Nocardia marina (Krassilnikov, 1949) 

 Waksman (Humm, H. J. and Shepard, K. 

 S. Duke Univ. Marine Sta. Bull. 3: 76, 

 1946; Krassilnikov, N. A., Guide to the 

 identification of bacteria and aetinomycetes, 

 Moscow, 1949). 



Synonyms: Proactinomyces flavus Humm 

 and Shepard. Proactinomyces citreus marinae 

 Krassilnikov. 



* Description after Erikson, D., Med. Res. 

 Council Spec. Rept. Ser., 203: 30, 1935. 



Morphology: Growth smooth, bright 

 yellow color, of a dough-like consistency. 

 Hyphae long, filiform, branching, breaking 

 down into short rods and cocci. Xo aerial 

 mycelium. 



Synthetic and protein salt water media: 

 Good growth. 



Gelatin liquefaction: Positive. 



Agar: Liquefied. 



Milk: No coagulation; peptonization posi- 

 tive. 



Nitrate reduction: None. 



Starch: Hydrolyzed. 



Carbon sources: Acetic, lactic, and butyric 

 acids utilized. Acid formed from various 

 sugars. 



Temperat ure : 25-30°C . 



Habitat: Atlantic Ocean marine deposit. 



35. Nocardia mesenterica (Orla- Jensen, 

 1919) Waksman and Henrici, 1948 (Orla- 

 Jensen, S. The lactic acid bacteria, 1919, 

 181 ; Jensen, H. L. Proc. Linnean Soc. N. S. 

 Wales 57: 373, 1932). 



Morphology: Growth forms extensive my- 

 celium composed of richly branching hyphae 

 of a somewhat variable thickness, 0.4 to 0.8 

 ll. No aerial mycelium. Later, hyphae divide 

 into fragments of varying sizes and shapes, 

 partly diphtheroid rods, but no real cocci. 

 There is, particularly in complex organic 

 media, a tendency to form large, swollen, 

 fusiform to almost spherical cells, up to 3.5 

 ix in diameter. These may stain intensely 

 with carbol fuchsin. 



Glucose-asparagine agar: Growth fair, 

 raised, granular, very pale yellow, glistening. 

 Condensation water-clear. 



Glucose-peptone agar: Growth excellent, 

 spreading. At first flat and smooth, pale 

 straw-yellow, perfectly hard and cartilagin- 

 ous, later raised and strongly folded, of a 

 loose, curd-like consistency, bright lemon- 

 yellow. 



Potato: Growth scant, restricted, soft, 

 cream-colored smear. 



Gelatin: Growth finely arborescent. 



