THE GENUS NOCARDIA 51 



much shorter than those of the latter. Erik- 40. Nocardia paraffinou (Jensen, L931) 



son (1949) added the following character- Waksman and Henrici, L948 (Jensen, II. 



istics: Soft cream to pink growth on nut ri- Proc. Linnean Soc. \. S. Wales .">(>: 362, 



enl agar media. On synthetic media, growth I!*.'!! I. 



colorless and thin, producing an initial myce- Morphology: Growth hard, firm, yellow- 



I iui ii, the hyphae dividing rapidly into short ish, consisting initially of an extensive myce- 



rods; addition of 0.01 per cent MnS0 4 stim- limn, with long, richly branching hyphae, 



ulated production of pale pink pigment. 0.4 to 0.5 ju thick. After 5 to 6 days, numer- 



Acid-fasl cell elements predominated during ous end branches swell to about double 



periods of maximum growth and tree air thickness, and divide into oval, spore-like 



supply. A study of the morphology of A', elements, 0.8 to 1.0 by 1.2 to 1.5 m- Division 



opaca grown on hydrocarbons and fatty starts at the tips of the swollen branches 



acids has been made by Webley (1955). and proceeds basipetally until most of the 



,_, .. , ... , hyphae appear divided. Primary septa have 



39. A ticiinha paniae (hnkson) Waksman • , , , , • ... 



,^ ., -r^ A,-, '-r, i ,, •. , ||()t been seen in the hyphae. 1 he spore-like 

 (Enkson, I). Med. Research Council Spe~ 



Kept. Ser. 203: L935, L6 17). 



Morphology: Substrate growth made up 

 of very small, round colonies; unicellular 

 mycelium with slender, branching filaments. 

 Aerial mycelium not visible on any medium 



elements are markedly acid-fast. Aerial my- 

 celium white consisting of short, straight, 

 not very much branched hyphae, 0.1 to 0.6 

 m thick, which never show any different iation 

 into spores (see also Krikson, 1949). 



, . , . ... " Sucrose nitrate agar: Growth very scant, 



but occasional isolated aerial branches. , . . . ., , . . .. * 



as thin colorless veil. Aerial nivcehuni trace. 



Glycerol nitrate agar: Growth poor; scant 

 colorless patch. 



Calcium malate agar: Growth colorless to 

 pink, spreading; later, brighl red mass, 

 buckled and shining, colorless submerged 

 margin. 



white. 



Glucose-asparagine agar: Growth fair, 

 hat, growing into medium ; pale ocher-yellow 

 to orange, with raised outgrowths on the 



surface. Aerial mycelium scant, white. 

 Nutrient agar: Growth slow, somewhat 



Nutrient agar: Growth irregularly piled . . ,. . . 



. . , ' . . . raised, ocher-yellow, hard, smeary >.... 



ip. convoluted, colorless, easily detachable, , . . , ,. ' ,, , . 



. , " loose. Aerial mycelium scant, small white 



brownisl 



Glucose agar: Small colorless coiled mass 

 later heaped up as green growth. 



Gelatin: Liquefaction rapid. 



Milk: Surface growth pale green. Coagula 

 t ion and peptonizat ion. 



Potato agar: Growth as small elevated 



t ufts. No soluble pigment . 



Potato: Growth mycelium granulated, 

 first pale yellow, later dee]) ocher-yellow to 

 orange. Aerial mycelium scant, white. No 

 soluble pigmenl . 



Gelat in: No liquefaction. 



Milk: No coagulation; no peptonization, 

 convoluted, colorless mass with purple tinge r,. . M , , , 



1 March: No hydrolysis. 



111 center - Cellulose: Not decomposed. 



Egg medium: Colonies small, round, Paraffin: Readily utilized, 



tough, colorless; margin well embedded. Nitrate reduction: Negative. 



Later, colonies elevated, waned, darkened. Sucrose: No1 inverted, 



medium discolored and broken. Slighl de- Liquid media (milk, broth, synthetic solu- 



gree of liquefaction; medium dark brown. tions): Small, round granules of various 



Source: An ulcer of the abdominal wall of yellow t ange color.-, firm but can be 



a patient in India. crushed into a homogeneous smear. In old 



