THK GENUS NOCARDIA 



53 



forms long wavy filaments, 0. 1 to 0.5 by 

 70 to LOO ft, extensively branched bu1 with- 

 out septa. Older cultures consist entirely of 

 rods, 4 to 10 ft, frequently in V-, Y-, or 

 smaller coccoid forms. Gram-positive, not 

 acid-fast, frequently showing hands and 

 granules. 



Nutrienl agar: Growth scant, orange-red. 

 No aerial mycelium. No soluble pigment. 



Glucose agar: Growth raised, flat, glisten- 

 ing, rose-colored, later becoming folded and 

 coral-red. 



Gelatin: Growth along stab thin, yellow- 

 ish, with thin radiating filaments. Surface 

 growth flat, wrinkled, red. No liquefaction. 



.Milk: Growth starts as small orange- 

 colored surface granules, later forming a 

 thick, soft, orange-colored sediment. No 

 coagulation; no peptonization. 



Starch: Hydrolyzed. 



Paraffin: Utilized. 



Cellulose: Xo growth. 



Temperature: Optimum 22-25°C. 



Source: Blood of a horse; soil in France 

 and Australia. 



Remarks: Differs from A'. r<>r<tllin<i in the 

 formation of very long filaments and in fili- 

 form growth in gelatin stabs. 



Type culture: IMRU 3409. 



4:>. Nocardia pulmonalis (Burnett, L909) 

 Waksman and Henrici, 1948 (Burnett, 



S. II. Ann. Kept. \. Y. State Vet. Coll. 

 1 909 1910, 107). 



Morphology: Mycelium acid-fast, espe- 

 cially in early stages of growth; breaks up 

 readily into oval-shaped cells. Growth 

 lemon-yellow with white aerial mycelium. 

 Consistency of colonies leathery. 



Peptone-beef extract agar: Growth moist, 

 raised, in form of small, spherical colonies. 



Glucose-peptone-beef extract agar: 

 ( irowth dull, whitish, convoluted. 



Potato: Growth abundant, in form of 

 small, translucent, round colonies, becoming 

 lemon-yellow. Later, growth becomes con- 



voluted or folded with chalky white aerial 

 mycelium. Color of plug brownish. 



Gelatin: Colonies small, whitish, spheri- 

 cal; edges of colony becoming chalky white. 

 Limited liquefad ion. 



Milk: Colonies on surface of the medium. 

 Coagulation and gradual peptonization. 



Pathogenicity: Nonpathogenic for rabbits 

 and guinea pig>. 



Source: Lungs of a cow. 



44. Nocardia rangoonensis (Erikson, 1935) 

 Waksman and Henrici, 1948 (Erikson, I). 

 Med. Research Council Spec. Rept. Ser. 

 203: :!•'! :!4, 1 <):;.")). 



Morphology: (irowth consists of branch- 

 ing hyphae which segment and present 

 slipping and angular arrangement. Aerial 

 hyphae few, short, straight, later developing 

 into a profusely branching, long, waving 

 aerial mycelium. Nol acid-fast. 



Glycerol nitrate agar: ( irowth dull, mealy, 

 pink, wrinkled. Aerial mycelium scant. 

 white. Medium slightly discolored. 



Nutrienl agar: Colonies round, lobate, 

 umbilicated, raised, cream-colored to pale 

 pink. Later, colonies colorless, medium dis- 

 colored dark brown. 



Glucose nutrient agar: (irowth con- 

 voluted, coherent, cream-colored; medium 

 discolored. Later, growth wrinkled, biscuit- 

 colored, colorless margin. Aerial mycelium 

 on border, white. Soluble pigment dark 

 brown. 



Potato agar: ( lolonies small, round, lemon- 

 colored, partly confluent. Submerged growth 

 greenish. Aerial mycelium white. Medium 

 colored light brown. 



Egg medium: (irowth extensive, colorless. 

 Aerial mycelium in center, pale pink. 



Gelatin: Colonies abundant, minute in 

 medium; larger, cream-colored colonic- on 

 surface. Aerial mycelium white. Brown pig- 

 ment surrounding growth. No liquefaction. 



Milk: Surface ring yellow. Coagulation 

 positive; peptonization partial. Soluble pig- 

 ment dark brown. 



