56 



THE ACTINOMYCETES, Vol. II 



«*.;*'■' 







Figure 15. A r . salmonicolor , growing on a hanging microdrop of liquid paraffin (a) surrounded by 

 sucrose nitrate salt solution; (b) same plus 1.5 per cent agar (Reproduced from: Webley, D. M. J. Gen. 

 Microbiol. 8: 71, 1953). 



Jong, 1927) Waksman and Henrici, 1948 

 (den Dooren de Jong. Centr. Bakteriol. 

 Parasitenk. Abt. II, 71: 216, 1927). 



Morphology: Growth made up of short 

 mycelium disintegrating into rods and cocci. 

 Aerial mycelium sometimes stretching into 

 quite long filaments, with small refractive 

 granules. Many cells at the edge of the 

 colonies show club- or pear-shaped swellings, 

 up to 2.5 to 8.0 /x in width; many of these 

 swollen cells later germinate with the forma- 

 tion of two more slender sprouts. Acid- 

 fastness is found among the earlier stages of 

 growth, especially in some of the strains and 

 on some media (Fig 15). 



Glucose-asparagine agar: Growth re- 

 stricted, rather flat, edges Lobate, surface 

 warty, glistening; at first pale orange, later 

 ocher-yellow; consistency crumbly. 



Glucose-peptone-beef extract agar: 

 Growth excellent, of a doughy consistency, 

 spreading, flat, dense, edges lobate, surface 

 folded, glistening, yellow, gradually chang- 

 ing to salmon-pink and deep orange-red. 



Potato: Growth good, raised, warty, 

 crumbly, glistening, at first buff, changing 

 to orange, and finally to almost blood-red. 



Gelatin: Growth in stab scant, arbores- 

 cent. Surface colonies small, wrinkled, 

 orange. No liquefaction. 



Milk: Pellicle of small cream-colored 

 granules, later a thick orange sediment. No 

 coagulation and no peptonization, although 

 milk appears slightly cleared, the reaction 

 becoming alkaline. 



Starch: Not hydrolyzed. 



Sucrose: Not inverted, although readily 

 utilized with sodium nitrate as a source of 

 nitrogen. 



Paraffin: Readily utilized as a source of 

 carbon. 



Cellulose: No growth. 



Phenol: Not utilized. 



Remarks: A detailed study of the acid- 

 fast properties of this species has been made 

 by Erikson (1949). It closely resembles N. 

 corallina. 



50. Nocardia 

 (Erikson, D. J. 

 393, 1954). 



Morphology: 

 acid-fast. Aei 

 characteristic 

 substrate and 



sebivorans Erikson, 

 Pathol. Bacteriol. 68: 



rram-pos 



lve, pa 

 white, 



1954 

 387- 



•tially 

 with 



ial mycelium 



nonwetting properties. Both 



aerial mycelium show spon- 



