THE GENUS NOCARDIA 57 



taneous segmentation into shorter and of small colonies, white at first, later be- 

 longer cells of coccoid or bacillary dimen- coming yellowish cream-colored, 

 sions. Biochemical properties: [ndole negative, 



Agar media: Colonies firmly attached to H 2 S positive, neutral red no1 reduced. Sugars 



medium. No soluble pigment. No acid pro- like glucose, sucrose, lactose, galactose, and 



duced from glucose, mannitol, lactose, mannose not attached. 



sucrose, starch, raffinose, galactose, rham- Milk: Coagulation positive; peptonization 



nose, sorbitol, maltose, dulcitol, glycogen, or positive, 



glycerol. Coagulated serum : Nol liquefied. 



Sucrose nitrate agar: Growth fair; nitrate Nitrate reduction: Positive. 



utilized. Oxygen demand: Strict aerobe. 



Gelatin: No liquefaction. Optimum temperature: 35 37 C. 



Carbon utilization : Paraffin is good source Pathogenicity: Pathogenic to guinea pigs 



of energy, also n-dodecane. Cresols not and rabbits. 



utilized. Habitat: Isolated from urine of patient 



Temperature: Can withstand exposure to suspected of renal tuberculosis. 



( .K)°C for 10 minutes in a phosphate buffer -.> v ,„.,.>• , ro •• i 



1 ' . , oZ. Nocardia sumatrae (Smiders emend. 



suspension; denser suspensions withstood 3 Erikson) (V em<cuH gnijd { .^ >k 



minutes a, LOO ( (Enkson, 1955). Tij(ls( . h X(i(1 ll|(|]( , f)J . 4 - ? 



Habital : Pus ma case oi empyema. X() , Streptothrix cuniculi Schmorl , 801 



Pathogenicity: Rabbits and gumea pigs, H()1 Nocardia mniculi ,,,. M( , |1() „„,, 



slightly for mice. Morphology: Growth made up of large 



Remarks: Cells have avidity for oily sub- ram ii flT1 ,., M . • ■ • ... . 



• • swollen cells, giving rise to ramifying fila- 



stances (lipophilic). I nder unfavorable con- ,„«,,* . , , , . u i • ,• i \ ■ , 



1 f ments or to small chains oi short, thick seg- 



ditions oi growth, on the surface oi solid , lwl ,, t . „.i- l i. i i 



, fe , ' , , , ments which branch out into more regular 



paraffin large clubs and hexagonal cells are hypha(> Sometimes the , n ,^ llal . elementg 



pr ° duced f lkS °"'rvr L* are besei with SI)i ">- processes befOTe g™g 



lype culture: N( K 8595. ,., S(1 |() lypi( . a , ,„„„. branching filaments . 



51. Nocardia serophila (Sartory and Later the picture becomes more mono- 



Bailly, L947) emend. Waksman (Sartory, A. morphous, and short straight aerial hyphae 



and Bailly, C. Compt. rend. 221: 1533-1534, are borne, which presently exhibit irregular 



1 *.)47 i . segmentation. 



Morphology: Hyphae produce angular Glycerol nitrate agar: Colonies small, 



growth, much branched, occasionally curved, round, elevated, cream-colored, margins 



04 to 0.5 n in diameter. Nonmotile. Rarely depressed; becoming smooth, discrete yel- 



certain secondary branches are spiral shaped, lowish. 



Terminal and intracellular arthrospores. ( rlucose-asparagine agar: ( Jolonies minute. 

 Gram-positive. Acid and alcohol resistant. colorless, becoming dull pink, partly con- 

 Growth characteristics: Crows with dim- fluent and piled up. Aerial spikes lew, stiff, 

 cully on ordinary solid or liquid media; pink- 

 grows well on serum or blood media. Nutrient agar: Colonies small, round, 

 Liquid peptone and serum media: Non- elevated, cream-colored, umbilicated and 

 viscous, cream-colored growth, detaches radially wrinkled. 



from tube by agitation, medium remaining Egg medium: Growth scant, pinkish, 



clear. smeary. 



Coagulated serum media: Growth in form Potato: Growth coral-pink, dry, granular, 



