58 



THE ACTINOMYCETES, Vol. II 



covered to a considerable extent with white 

 aerial mycelium; piled up in center, discrete 

 colonies at margin. 



Gelatin: Few flakes. No liquefaction. 



Milk: Heavy yellow growth attached to 

 walls; solid coagulum in 1 month. 



Nutrient broth: Surface colonies cream- 

 colored, scale-like; abundant, flocculent 

 bottom growth. 



Source: Infected rabbits. 



Remarks: Description given after Erikson 

 (id:;;,.. 



53. Nocardia transvalensis Pijper and 

 Pullinger, 1927 (Pijper, A. and Pullinger, 

 B. D. J. Trop. Med. Hyg. 30: 153, 1927). 



Morphology: Initial growth made up of 

 unicellular hyphae, the central branch being 

 frequently broader and showing dense 

 granular retractile contents. Aerial mycelium 

 white, forming straight hyphae, in some 

 cases becoming clustered into irregular 

 spikes. ( 'dlorless drops are exuded and a pink 

 coloration is produced in the densest part of 

 the growth on synthetic glycerol agar. 

 Angular branching with division of substrate 

 filaments. Aerial hyphae irregularly seg- 

 mented. Acid-fast. 



Glycerol nitrate agar: Growth in form of 

 small, pink coiled masses. Aerial mycelium 

 thin, white. 



Nutrient agar: No growth. 



Glucose nutrient agar: Colonies raised, 

 granular, pink. Aerial mycelium white. 



Potato: Growth dry, raised, convoluted, 

 pink. Aerial mycelium white. 



Gelatin: Growth poor, in form of a few 

 irregular, colorless flakes. No liquefaction. 



Milk: No change. 



Starch: Not hydrolyzed. 



Egg medium: Growth in form of small, 

 irregularly raised, coiled, dull pink mass. 



Source: A case of mycetoma of the foot , in 

 South Africa. 



Pathogenicity: To guinea pigs. 



Remarks: According to Gonzalez Ochoa 



and Sandoval (1956), A', transvalensis is a 

 synonym of N. brasiliensis. 



54. Nocardia turbata Erikson, 1954 (Erik- 

 son, D. J. Gen. Microbiol. 11: 198-208, 

 1954). 



Morphology: Typical actinomycete, pro- 

 ducing a fine mycelium composed of slender 

 filaments, 0.1 /j. in diameter, which fragment 

 into rods and coccoid cells. Under appro- 

 priate conditions, many cells are motile. 

 Nonacid-fast. 



Agar media: Growth good. Colonies small, 

 0.1 to 2.0 n. Initially colorless, later pro- 

 ducing a yellow-green pigment on nutrient 

 agar. Pigment production favored by free 

 air supply, suppressed by acid reaction. 



Broth cultures: Turbid when young; sedi- 

 mentation of cells later, when pellicle and 

 clarification of medium produced. 



Acid production: Positive with glucose, 

 sucrose, maltose, lactose, galactose, xylose, 

 arabinose, glycerol, starch; negative with 

 mannitol, raffinose, rhamnose, sorbitol, 

 dulcitol (using a casein hydrolysate me- 

 dium). 



Oxygen demand: Aerobic. 



Optimum temperature: 20-30°C. 



Nitrate: Utilized. 



Gelatin: No hydrolysis, except in presence 

 of peptone (slowly). 



Paraffin utilization: Negative. 



Habitat : Probably soil. 



55. Nocardia uniformis Marton and 

 Szabo, 1959 (Mail on, M. and Szabo, I. 

 Acta Microbiol. Acad. Sci. Hung. 6: 131- 

 135, 1959). 



Morphology: The filaments of the sub- 

 strate mycelium rapidly break up into rods 

 and less frequently into coccoid bodies. The 

 size of these forms is 0.7 to 1.1 m by 1.1 to 

 4.0 /j.. In old cultures, swollen, club- or bol tie- 

 shaped forms appear. The hyphae of the 

 slightly developed aerial mycelium are 

 straight or waxed, nonseptate, and contain 



