GROUPS AND SPECIES OF GENUS STREPTOMYCES 

 red, aerial mycelium 



^7 



I). (Irowth rose c 



rosy. 



Actinomyces Fradii 



c. Growth a mixture of white and yel 

 low. 



a 1 . No conidia. 



Actinomyc* s albo flavus 

 b 1 . Abundanl conidia. 



a 2 . Conidia mil shaped, powdery, 

 gray-yellow. 

 Actinomyces tins* us 

 b 2 . Conidia spherical ami oval, 

 grow th compact . cit ron-yel- 

 low. 

 Actinomyces citreus 



d. Growth at firsl colorless, then brown 

 to black. 



a 1 . Aerial mycelium white. 



Actinomyces alboatrus 

 1)'. Aerial mycelium dark gray. 

 Actinomyc* s Lipmanii 

 2. Soluble pigment produced. 

 a. Soluble pigment green. 



Actinomyc* s Verm 

 1). Soluble pigment dark blue. 



Actinomyces violaceus niger 

 B. Gelatin rapidly liquefied; brown pigment 



I. Spirals produced. 



a. Growth rose-colored; aerial mycelium 



rosy. 



Actinomyces ros* us 



b. Growth colorless; aerial mycelium 

 golden brown. 



Actinomyc* s aureus 

 c Growth slightly brown; aerial mycel 



ium white. 



Actinomyces Halstedii 

 II. Xo spirals. 



1. No soluble pigmenl on synthetic media. 

 a. Growth red to red orange; no aerial 



mycelium. 



Actinomyces Bobili 

 1 1. Growth white; aerial mycelium white. 



a 1 . Aerial mycelium thin. rare, net 

 like. 



Actinomyces reticuli 

 b 1 . Aerial mycelium thick, white to 

 gray. 



Acti mull yet s albus 



2. Soluble brown pigment produced on 

 SJ n! het ic media. 



a. Aerial mycelium white, abundant. 



Actinomyces diastato chromo- 



iji n us 



b. Aerial mycelium white, produced 



late or not at all. 



1 1 tinomyc* s chromog* u us 



group 



c. < bow t h green ; aerial mycelium \\ lute. 



Actinomyces virido chromo- 



IJI II us 



('. Gelatin slowlj liquefied; oo soluble pigmenl 

 1. Spiral- produced in aerial mycelium. 



1. Soluble led and blue pigment-. 



Actinomyces viola* * us 



2. No soluble pigment; substrate growth 

 red. 



Actinomyces < 'alifornicus 

 II No spirals produced in aerial mycelium. 



1 . Growth yellow; no soluble pigmenl 



Actinomyces parvus 



2. Growth tends to crack; soluble brown 



pigmenl . 



Actinomyc* s < xfoliatus 

 1). Gelatin slowly liquefied; brown pigment pro- 

 duced. 

 I. Spirals produced; aerial mycelium laven- 

 der. 



.1 eh limn i/ri s Inn ml ulm 

 II. Xo spirals. 



1. Growth yellow; aerial mycelium gray. 



Actinomyces Jim us 



2. Growth colorless; aerial mycelium 



purplish while. 



Actinomyc* s purpurog* nus 

 :!. Growth black; aerial mycelium scant. 

 Actinomyces i ith rochromo- 

 genus 



1. ( irowth purple; no aerial mycelium. 



Actinomyc* s purp* o chromo 



I It It us 



2. WAKSMAN SYSTEM (1919) 



This was a modification of the previous 

 system and was based upon a study of 11 

 species. An examination was made of the 

 morphology of the aerial mycelium on two 

 media; growth, aerial mycelium, and solu- 

 ble pigmenl <>n 12 different media; various 

 biochemical properties, such as carbon and 

 nitrogen utilization, proteolytic activities, 

 diastase and invertase formation, reduction 

 of nitrate to nitrite, and change in reaction 

 of medium. A brief outline is presented here: 

 A. Soluble pigmenl produced on organic 

 media. 



