GROUPS AM) SPECIES OF GENUS STREPTOMYCES 



9.3 



be subdivided 



5is of spiral 



FLAIG WD KUTZNEB SYSTEM (1954, I960) 



Flaig and Kutzner (1954) and Kutzner 

 ui 2000 Sin ptomyces cul- 

 i were authentic species 

 soil isolates. They charac- 

 •es by several criteria in- 

 laracterisl ics on complex 

 dia, morphology of the 

 physiological properl ies, 

 vity against five test or- 



- L956) studied 



tares, 63 of \ 



and the rest t'l 



terized their <• 



eluding cultun 



and synthetic 



aerial myceliu 



and antibiotic 



ganisms. On the basis of these studies, a 



key was prepared. At first the material was 



divided into "groups" on the basis of the 



color of the aerial mycelium on oatmeal 



agar, six spore colors being recognized. The 



gray color group was further divided, on the 



basis of the color of the substrate mycelium 



and soluble pigmenl on this medium, thus 



resulting in the 10 groups shown in Table <). 



batei', however, Flaig and Kutzner (1960) 

 reached the conclusion that the subdivision 

 of the gray-spored color group may lead to 

 difficulties when new isolates have to be 

 placed in one of these groups, although this 

 system proved to be quite useful iii studying 

 many si rains at t he same t ime. 



Each group was further divided into sub- 

 groups (altogether 382) on the basis of pig- 



menl formation on glucose-peptone agar, 

 morphology of the aerial mycelium, anti- 

 biotic activity againsl five tesl organisms, 

 and cultural characteristics on several 

 media. The shape of the spores (observed 

 with electron microscope) was given for 17.~> 

 of the 382 subgroups. Various authentic 

 species were included in these subgroups 

 according to their characteristic properties. 

 'Hie following species were placed in the 

 various groups. 



Group I: S. albus, S. griseus, S. chryso- 

 mallus, S. coelicolor, S. cali- 

 fornicus 

 Group II: S. longispororuber, S. bobiliae, 

 S. roseochromogenes, S. Vene- 

 zuela?, S. phaeochromogenes 

 Group III: S. lavendulae, S. xanthophaeus 

 Group IV: S. flavogriseus, S. globisporus, 



S. flaveolus 

 Group V: S. diastaticus, S. globosus 

 Group VI : S. flavus 



Group VII: S. craterifer, S. griseolus, S. 

 halstedii, S. hygroscopicus, S. 

 aun ofacit us, S. violaceorubi r 

 Group Mil: S. purpurascens 

 Group IX: S. cyaneus, S. viridochromo- 



(/< nes, S. chartn usis 

 ('■roup X: N. hirsutus, S. prasinus, S. 

 prasinopilosus 



Table 9 

 Grouping of iht genus Streptomyces according In characteristic coloration (Flaig and Kutzner. I960 



Group 



Aerial myielium 



Substrate mycelium and soluble pigmenl 



Colorless, brownish, reddish, greenish 

 Colorless, orange, greenish, brownish to 



dark brown, pink to dark red 

 Colorless, orange, brownish to dark brown 

 Yellowish-green to green 

 I') row ii 



( lolorless, brownish to brow d 

 Colorless, greenish gray, brownish gray, 



orange-brownish, red or blue violet 

 \ Lolel 



Blue-purple, bluish-green, brownish 

 Colorless, greenish, brownish to rose-red 



