94 



THE ACTINOMYCETES, Vol. II 



3. SYSTEM OF YAMAGUCHI AND SABURI (1955) 



Yamaguchi and Saburi also used morpho- 

 logical features as the primary basis for the 

 separation of the genus Streptomyces into 

 groups, and physiological characteristics 

 for further separation. Although they were 

 concerned primarily with species of Strepto- 

 myces possessing antitrichomonal properties, 

 their system may apply to the genus as a 

 whole. 



I. Sporophores straight, tuft -forming tendency 

 in the margin; no verticils or spirals. 



1. Aerial mycelium gray. 



a. Soluble pigment on protein media. 

 Light purple, reddish-purple, purplish- 

 brown, sometimes yellowish-brown. 



St reptom yces p u rpeojusc us 



b. Xo soluble brown pigment. 



Streptomyces fasciculus 



2. Aerial mycelium pale yellowish-green. 



a. Soluble pigment on protein media 

 brown— ATCC Culture No. 3309. 



b. Soluble pigment brown. 



Streptomyces griseus 

 II. Sporophores straight, verticils produced. 

 1. Cottony aerial mycelium white, light tan, 

 or pale pink. 



a. Soluble pigment on protein media 

 brown. 



Streptomyces reticuli 

 1). Xo soluble brown pigment on protein 

 media. 



Streptomyces hack ijoensis 

 III. Sporophores spiral-shaped. 



1. Predominantly closed spirals produced. 



a. Aerial mycelium gray. 



a 1 . Soluble brown pigment on protein 

 media. 



a 2 . Growth colorless, yellowish- 

 brown, brown, or deep brown. 

 Streptomyces olivochromogenes 

 b 2 . ( rrowth light purple to purplish- 

 black. 



Streptom yces p u rp u reoch ro - 

 mogenes 

 b 1 . Xo soluble brown pigment. 



Streptomyces o urcofocicus 



b. Aerial mycelium pale pink. 



a 1 . Soluble pigment on protein media 

 brown. 



Streptomyces lavendulae 

 I) 1 . Xo soluble brown pigment. 



Streptomyces fradiat 



2. Predominantly open spirals or compact 

 spirals produced. 



a. Aerial mycelium white. 



a 1 . Soluble pigment on protein media 

 brown. 

 a 2 . ( rrowth reddish. 



Streptomyces ruber 

 b 1 . Xo soluble brown pigment. 

 Streptomyces farinosus 



b. Aerial mycelium gray. 



a 1 . Soluble pigment on protein media 

 brown. 



a 2 . Abundant compact spirals pro- 

 duced on aerial hyphae. 

 Streptomyces naganishii 

 b 2 . Growth colorless, white, light 

 yellow, or yellowish-brown. 

 Streptomyces diastatochromo- 

 genes 

 c 2 . Growth colorless, reddish-or- 

 ange, or reddish-purple. 

 Str< ptomyces griseoruber 

 b 1 . Xo soluble brown pigment. 

 a'-'. Growth colorless to creamy. 



Streptomyces albus 

 b 2 . Growth colorless, white, light 

 yellow, or yellowish-brown. 

 G 167 (resembling Strepto- 

 myces cacaoi) 

 c'-. Growth colorless, light yellow, 

 or light pinkish-brown. 



Streptomyces albogriseolus 

 d 2 . Growth purple to pink to red. 

 Streptomyces calif orn if us 



c. Aerial mycelium gray, but on certain 

 media moist with dark, glistening 

 patches. 



Streptomyces hygroscopicus 

 IX. Xo characteristic features of aerial hyphae. 



1. Very limited aerial mycelium production 

 on various media. 



a. Soluble pigment on protein media dee]) 

 brown. 



a 1 . Growth yellow to yellowish-brown. 

 Streptomya s flavochromogi m s 



b. Soluble pigment faint yellowish-brown. 



Streptomyces thioluteus 



2. Aerial mycelium white. 



a. Growth colorless, light yellow, or red 

 dish-orange. 



Streptomyces ruber 



4. BALDACC1 SYSTEM (1956, 1958, 1959) 



Following the example of Sanfelice and 

 Waksman, Baldacci divided the genus 



