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THE ACTINOMYCETES, Vol. II 



Table 21 



Classification of actinomycin-producing organisms based upon utilization of rhamnose and 

 raffinose (Ettlinger et al., 1956) 



Table 22 



Grouping of actinomycin-producing organisms (Ettlinger el al., 1956) 



tion and regeneration of S. griseus was made 

 by Williams and McCoy (1953). 



Different varieties of S. griseus vary 

 greatly in their cross-resistance and in their 

 sensitivity to actinophages. 



It must further he noted that high-yielding 

 streptomycin strains can he obtained by 

 irradiation, by growth in media containing 

 increasing concentrations of streptomycin, 

 and by strain selection. 



Okami (1950a) examined 47 strains be- 

 longing to the Griseus series. Five of these 

 were grisein-forming strains and four were 



pink pigmented forms. The streptomycin- 

 producing strains grew in maltose-containing 

 media with NaN0 3 as a source of nitrogen, 

 but not in glucose, glycerol, or sucrose media. 

 The grisein strains grew in media containing 

 any of the four carbon compounds. The pink 

 strains grew only in glycerol media (Table 

 23). The utilization of the carbohydrate was 

 found to depend largely on the nitrogen 

 source. In the presence of ammonium sulfate, 

 the above differences disappeared. All strains 

 utilized xylose, but not raffinose or rham- 

 nose. Sensitivity to phage was said to be 



