DKSCKII'TIOX OF Sl'IX'IKS OF STREPTOMYCES 



L67 



Morphology: Sporophores straight, qo 

 spirals. Spores cylindrical. 



Glycerol nitrate agar: Growth colorless to 

 brownish. Aerial mycelium scant, white to 

 dark grayish. Soluble pigmenl brown. 



Glucose-asparagine agar: Growth yellow- 

 ish brown. Aerial mycelium scant, yellowish- 

 white. Soluble pigmenl none or slightly 

 brown. 



Nutrient agar: Growth wrinkled, elevated, 

 colorless to brownish. No aerial mycelium 

 or soluble pigmenl . 



Potato: Growth yellowish-brown to 

 brownish, fine, wrinkled. Aerial mycelium 

 white, powdery. Soluble pigment absent at 

 first, later reddish-brown. 



Gelatin: Growth yellowish-brown. Soluble 

 pigment slightly brown. Liquefaction very 

 weak. Melanin-negative. 



Milk: Surface growth poor. No soluble pig- 

 ment. Coagulation and slow peptonization. 



Egg media: Growth reddish-brown, 

 wrinkled. No aerial mycelium. No soluble 

 pigment. 



Nitrate reduction: Positive. 



Antagonistic properties: Produces an anti- 

 viral agent, achromoviromycin. 



Remarks: This culture resembles S. 

 diastaticus and S. fimicarius. It is character- 

 ized by the brown pigmentation on syn- 

 thetic agar only. A strain of this organism, 

 which produces the antibiotic streptozotocin, 

 was isolated by Vavra et ol. (1959) from a 

 soil in Kansas; they have further cultural 

 data concerning the original culture and the 

 new strain. 



Type culture: [MRU 3730; ATCC 12,767. 



4. Streptomya s acidom ycet icus < >gata < / al., 

 L954 (Ogata, K., Miyake, A., and Morimoto, 

 A. Japanese Patent No. 204,403, March 5, 

 L954). 



Morphology: Sporophores usually do not 

 form spirals. Spores cylindrical or oval, 

 0.8 to 1.2 by 1.1 to l.S M . 



Sucrose nitrate agar: Growth at firsl 

 light yellow, later dark greenish-brown. 



Aerial mycelium greenish to yellow-white. 



Soluble pigmenl slightly violet -colored : 

 sometimes absent. 



Glucose-asparagine agar: Growth brown- 

 ish-yellow or brownish-red. and partially 

 greenish-blue. 



Calcium malate agar: Aerial mycelium 

 greenish-white. Soluble pigmenl violet; 

 sometimes absent . 



Glucose nutrient agar: Growth brownish, 

 partially dark blue. Aerial hyphae gray- 

 white. Soluble pigment brownish-black. 



Gelatin: Growth dark green. Aerial my- 

 celium greenish-white. Soluble pigment 

 greenish-brown. Liquefaction limited. 



Potato: Growth greenish-brown. Aerial 

 mycelium at first white, later pinkish-red. 

 Soluble pigment dark green. 



Milk: Growth cream-colored, later turning 

 light brown. No coagulation. Soluble pig- 

 ment light brown. 



Starch: Slow decomposition. 



Tyrosinase reaction: Negative. 



Nitrate reduction: Positive. 



Production of IPS: Positive. 



Carbon utilization: Arabinose, glucose, 

 maltose, lactose, salicin, and salts of or- 

 ganic acids utilized. Xylose, fructose, raffi- 

 nose, inulin not attacked. 



Antagonistic properties: Produces the 

 antibiotic acidomycin. 



Remarks: S. acidomyceticus is closely re- 

 lated to S. phaeochromogenes, the latter 

 forming spirals in gelatin media, but not the 

 former. 



Type culture: ATCC 11,611. 



5. Streptomyces acidophilus (Jensen, L928 

 Waksman and Henrici, 1 ( .)4S (Jensen, II. L. 

 Soil Sci. 25: 226, L928). 



Morphology: Sporophores either few or 

 numerous, with sinistrorse spirals. Spores 

 oval and spherical, L.O to 1.2 by 1.2 to L.5 



M- 



Agar media: Growth on acid media (pll 

 2.0 to 6.0) colorless. Aerial mycelium whitish. 



Sucrose nitrate agar: No growth. 



