DESCRIPTION OF SPECIES OF STREPTOMYCES 



171 



mycelium white. Soluble pigment dark. 

 greenish-black. 



Gelatin: Growth fair. Aerial mycelium 

 while. Soluble pigmenl lighl yellow. Lique- 

 facl ion medium. 



Milk: Surface white ring, with yellow- 

 green to lighl yellow-brown below surface. 

 Aerial mycelium white. Peptonization slow. 



Cellulose: No growth. 



Production of IPS: Negative. 



Antagonistic properties: Produces puro- 

 mycin, an antibiotic active upon certain 

 gram-positive bacteria and protozoa. 



Habitat : Forest soil. 



Remarks: Culture is characterized by the 

 formation of an olivaceous black soluble 

 pigment in some media, such as asparagine- 

 glucose agar, but no such pigment is pro- 

 duced on certain organic media. 



Type culture: ATCC 12,461. 



13. Streptomyces albosporeus (Krainsky, 

 1014) Waksman and Henrici, 1948 (Krain- 

 sky, A. Centr. Bakteriol. Parasitenk. Abt. 

 [I., 41: 687, 1014; Waksman, S. A. and 

 Curtis, II. ]•:. Soil Sci. I: 00, 1916; 8: 90, 

 I 'J I!) i. 



Morphology: Sporophores straight, 



branching', with occasional spirals. Spores 

 spherical or oval, 0.8 to 1.2 by 1.0 to 1.8m- 



Sucrose nitrate agar: Growth spreading, 

 colorless, with pink center, becoming brown- 

 ish, vinaceous. Aerial mycelium white, 

 covering the whole surface; often none. No 

 soluble pigment. 



Glycerol malate agar: Growth rose to 

 orange-red. Aerial mycelium white, later 

 changing to yellow. No soluble pigment. 



( rlucose-asparagineagar:( Growth wrinkled, 

 spreading, red, with colorless margin. Aerial 

 mycelium appears late, white. 



Nutrient agar: Small, cream-colored col- 

 onies. No aerial mycelium. No soluble 

 pigment. 



Starch agar: Growth thin, -pleading. 

 transparent, with red tinge. No aerial 

 mycelium. Heady hydrolysis. 



Potato: Growth red to brownish-gray. 

 No aerial mycelium, or white. Melanin- 

 negative. 



Gelatin: Growth yellow, changing to red, 

 with hyaline margin. Usually no aerial my- 

 celium; when produced, sometimes gray. 

 Medium liquefaction. 



Milk: Scant, pink ring. No coagulation; 

 no peptonization. 



Cellulose: No growth or scant. 



Nitrate reduction: I'air. 



Production of IPS: Negative. 



Temperature: Optimum :!7°C. 



Antagonistic properties: Positive. 



Habitat : Soil. 



Remarks: Above description is based 

 partly upon the isolates of Waksman and 

 Curtis, since Krainsky's culture was not 

 available. Krassilnikov (1040) considered it 

 as a variety of A. ruber. According to 

 Ettlinger et al. (1958) this organism should 

 be considered as a strain of S. griseus, a 

 hardly justifiable assumption. 



Type culture: [MRU 3003. 



14. Streptomyces alboviridis (I)uche, 1934) 

 Waksman ( I )uche, J. Pes actinomyces du 

 groupe albus. P. Lechevalier, Paris, p. :!I7, 

 1934). 



Morphology: According to Krassilnikov 

 ( 1949), the sporophores produce spirals with 

 3 to 4 turns. Spores spherical. 



Glucose nitrate agar: Growth cream- 

 colored becoming olive-green. Aerial myce- 

 lium white, becoming yellowish-green. Solu- 

 ble pigment brownish. 



Glucose-asparagine agar: Growth at first 

 white, becoming olive-colored to almost 

 dark. Aerial mycelium white to green. Solu- 

 ble pigment yellowish. 



Starch agar: Growth cream-colored: re- 

 verse brownish-green. Aerial mycelium 

 white, becoming green. 



Gelatin: Growth white, becoming green. 

 Soluble pigment greenish-brown. Liquefac- 

 tion rapid (slow, according to Krassilnikov, 

 L949). 



