DESCRIPTION OF SPECIES OF STPEPTOMYCKS 



17:; 



Aerial mycelium while to 

 c pigment. No hydrolysis in 



Glucose-asparagine agar: Growth color- 

 less to white, later lighl brown with or 

 without dull lighl reddish tinge. Surface 

 "lossy. Aerial mycelium scant, white. Light 

 brown to dull light reddish-brown soluble 

 pigment. 



Starch agar 

 gray. No solul 

 7 days. 



Glucose-asparagine agar: Growth color- 

 less to while, later lighl brown with or with- 

 out dull lighl reddish tinge. Surface "lossy. 

 Aerial mycelium scant, white. Lighl brown 

 to dull lighl reddish-brown soluble pigment. 



Glucose nutrieni agar: Growth lighl 

 yellowish-brown. Surface glossy. Aerial 

 mycelium white to gray. Soluble pigmenl 

 lighl yellowish-brown. 



Potato: ( trowth abundant , lighl yellowish- 

 brown. Aerial mycelium white to gray. 



Gelatin: Scant growth. No liquefaction. 

 Brown soluble pigmenl . 



Milk: Lighl yellowish-brown surface ring, 

 with scant white aerial mycelium. Yellow- 

 ish-orange soluble pigment occasionally. 

 Peptonization positive. 



Egg medium (37°C) : Growth yellow with 

 gray tinge. Aerial mycelium white, later 

 lighl purplish occasionally. 



Cellulose: Scant growth with purplish- 

 gray aerial mycelium and lighl purplish 

 pigmenl . 



Carbon utilization: Abundant growth with 

 rhamnose, fructose, galactose, mannitol, and 

 glucose; weak growth with xylose, arabinose, 

 maltose, sorbitol, and inositol; none or 

 very scant with dulcitol, raffinose, and 

 inulin. 



Antagonistic properties: Produces anti- 

 biotic althiomycin. 



Remarks: Closely related to S. achromo- 

 genes and S. rimosus. Spiral formation of 

 culture, no nitrile formation, and purplish 

 tone of growth and aerial mycelium differ- 

 entiate ii from S. achromogenes. Purplish 

 tinge of aerial mycelium and growth, soluble 



pigment, no nitrite formation, and no 

 cracked surface of the growth differentiate 

 it from S. rimosus. 



17. Streptomyccs ambofaciens Pinnert-Sin- 

 dico, 1954 (Pinnert-Sindico, S. Ann. inst. 

 Pasteur 87: 70:5 707, 1954). 



.Morphology: Sporophores form spirals. 

 Spores oval or spherical. 



Sucrose nitrate agar: Substrate growth 

 yellow to gray. Aerial mycelium white to 

 gray. Soluble pigment weak brownish-yel- 

 low. 



Glucose-asparagine agar: Growth yellow, 

 covered with white aerial mycelium. Soluble 

 pigment weak yellow-brown. 



Calcium malate agar: Growth similar to 

 that on sucrose nitrate agar. No soluble 

 pigment. 



Potato: Growth clear brown. Aerial 

 mycelium powdery gray. Soluble pigmenl 

 weakly blown to brownish-red. 



Gelatin: Surface growth yellow; flakes in 

 liquefied portion. Medium liquefaction. 

 Weak brown-orange pigment in liquefied 

 zone. Melanin-negative. 



Milk: No coagulation, partial peptoniza- 

 tion in 1 month. Peptonized zone orange- 

 brown to red. 



Nitrate: Weak reduction to nitrite in 

 synthetic media; none at all in organic 

 media. 



Production of IPS: Negative. 



Carbon utilization: Glycerol, arabinose, 

 glucose, galactose, levulose, mannose, lac- 

 tose, rhamnose, starch, and mannitol well 

 utilized. Raffinose, erythritol, dulcitol, and 

 sorbitol not utilized. 



Antagonistic properties: Produces two 

 antibiotics, congocidin and spiramycin. 



Remarks: Ettlinger et al. (1958) included 

 this organism with S. aureofaeiens. 



18. Streptomyces annulatus (Beijerinck, 

 1912; emend. Krassilnikov, 1941) Waksman 

 ( Krassilnikov, N. A. Actinomycetales. [zvest. 

 Akad. Nauk, SSSR, Moskau, PHI). 



