176 



THE A.CTINOMYCETES, Vol. II 



spirals; individual filaments arc rarely sep- 

 tate. Sporophores straight, flexuous, or 

 fascicled (in tufts). Spores are oval to oblong, 

 1.0 to 1.2 ix. The spore color is light gull-gray. 



Sucrose nitrate agar: Xo growth. 



Nutrient agar: Growth colorless, abun- 

 dant, spreading. Aerial mycelium white. No 

 soluble pigment. 



Oatmeal agar: Growth good. Aerial myce- 

 lium limited, no sporulation. Soluble pig- 

 ment slight maize-yellow. 



Casein digest-meat extract agar: Growth 

 abundant, dark olive-buff. Aerial mycelium 

 well developed, pale smoke-gray. No soluble 

 pigment. 



Gelatin: Rapid liquefaction. Melanin-neg- 

 ative. 



Milk: Positive coagulation and peptoniza- 

 tion. 



Potato: Growth good, creamy-buff, cere- 

 briform. Aerial mycelium white; no sporula- 

 tion. No soluble pigment. 



Starch: Hydrolyzed. 



Nitrate reduction: Positive. 



Carbon utilization: Mannitol, d-xylose, 

 Z-arabinose, /-rhamnose, d-fructose, treha- 

 lose, and lactose utilized. No growth or very 

 scant growth with inositol, sorbitol, meli- 

 biose, sucrose, and dextrin. 



Habitat: Soil. 



Biochemical activities: Certain strains of 

 this organism convert progesterone to 10 

 a-hydroxyprogesterone. 



2:!. Streptomyces armillatus Mancy-Cour- 

 tillet and Pinnert-Sindico, 1954 (Mancy- 

 Courtillet, I), and Pinnert-Sindico, S. Ann. 

 inst. Pasteur 87: 580-584, 1954). 



Morphology: Aerial mycelium produces 

 spirals. 



Glucose- or glycerol-asparagine agar: 

 Growth yellow-gray. Aerial mycelium poorly 

 developed, white. 



Sucrose nitrate agar: Growth very poor, 

 colorless. No aerial mycelium. 



Glucose nitrate agar: Growth pool'. No 

 soluble pigment. Xo reduction of nitrate. 



Glucose-peptone agar: ( rrowth very good, 

 yellow-gray. Aerial mycelium poorly devel- 

 oped, white. Soluble pigment weak rose, 

 becoming brownish. Melanin-negative. 



Potato: Growth good, yellow-gray. Aerial 

 mycelium poorly developed. No soluble 

 pigment. 



Tyrosine medium: Growth flat, yellow- 

 gray, becoming beige. Aerial mycelium 

 white. Xo soluble pigment. 



( lelatin: Growth in form of pellicle. Aerial 

 mycelium white. Soluble pigment rose- 

 brown. Rapid Liquefaction. 



Milk: Growth in form of surface ring, 

 gray to yellow. Peptonized portion colored 

 yellow. Coagulation and rapid peptoniza- 

 tion. 



Starch: Xo hydrolysis. 



Antagonistic properties: Produces oxytet- 

 racycline. 



Remarks: This organism can be classified 

 with the S. bobiliae-S. erythreus group, al- 

 though its growth is yellow rather than red. 

 It grows poorly upon synthetic media, upon 

 which it forms no aerial mycelium. It does 

 not reduce nitrate to nitrite. It does not 

 produce a purple pigment upon egg media. 

 It docs not change the reaction of milk to 

 alkaline. It does not hydrolyze starch. It 

 differs from S. rimosus and S. griseoflavus, 

 which produce yellow to brown pigments; 

 S. armillatus under the same conditions does 

 not form any pigment. 



24. Streptomyces aurantiacus (Rossi- 

 Doria, 1891 emend. Gasperini, 1892; emend. 

 Krassilnikov) Waksman (Krassilnikov, X. 

 A. Actinomycetales. Izvest. Akad. Xauk. 

 SSSR, Moskau, p. 36, 11)41). 



Morphology: Produces an abundance of 

 chlamydospores. Sporophores form spirals 

 with 3 to 5 turns. Spores spherical to oval. 

 0.7 to ().<) by ()A\ to 0.8 m. 



Agar media: Growth lichenoid, dry, com- 

 pact; colored bright orange or golden. Pig- 

 ment insoluble in medium, but soluble in 



