180 



THE ACTINOMYCETES, Vol. II 



Starch agar: Growth spreading, colorless. 

 Aerial mycelium abundant, white. Hydroly- 

 sis. 



Egg medium: Growth extensive, wrinkled, 

 bright yellow. Considerable liquefaction. 



Blood agar: Growth in uniformly striated 

 colorless bands; occasional round colonies at 

 margin. Hemolysis positive. 



Potato: Growth moist, colorless. Aerial 

 mycelium scant, white at top of plug. 



Gelatin: Dull white flakes sinking to bot- 

 tom as medium liquefies. Rapid liquefaction. 



Milk : Coagulation followed by peptoniza- 

 tion. 



Source: Human spleen in a case of splenic 

 anemia. No record concerning actual patho- 

 genicity. 



28a. Streptomyces bellus Margalith and 

 Beretta (Margalith, P. and Beretta, G. 

 Mycopathol. Mycol. Appl. 12: 189-195, 

 1960). 



Morphology: Sporophores long, straight, 

 flexuous with short, open hooks. 



Sucrose nitrate agar: Substrate growth 

 light cherry-pink. Aerial mycelium pinkish- 

 white. Soluble pigment light pink. 



Glucose-asparagine agar: Growth colorless 

 to light pink with orange tinge. Aerial myce- 

 lium white, with small amount of bluish 

 spores. Soluble pigment light pink with 

 orange tinge. 



Calcium malate agar: Substrate growth 

 pink, with pinkish-violet reverse. Sporula- 

 tion abundant, pinkish-blue. Soluble pig- 

 ment pinkish-violet. 



Nutrient agar: Substrate growth hyaline 

 with brownish reverse. Aerial mycelium 

 slight or absent. Soluble pigment brown. 



Starch agar: Growth hyaline with color- 

 less to pale pink reverse. Aerial mycelium 

 limited, with bluish-green spores. Starch 

 hydrolyzed. 



Glucose-casein digest-yeast-beef agar: 

 Growth pink. Aerial mycelium pinkish with 

 bluish-green spores. Soluble pigment light 

 pink. 



Glucose-yeast extract-beef-peptone agar: 

 Growth pink. Aerial mycelium pinkish- 

 white with bluish-green spores. Soluble pig- 

 ment reddish-brown. 



Potato: Growth rough, colorless to brown. 

 No aerial mycelium. Soluble pigment brown. 



Gelatin: Slow liquefaction. Soluble pig- 

 ment brown. 



Milk: Growth in form of brownish ring. 



Nitrate reduction: None. 



Cellulose: Moderate growth. 



Carbon utilization: Most sugars readily 

 utilized. Xylose, inulin, and dulcitol not 

 utilized in solid media. Succinate, citrate and 

 glycine not utilized. 



Antagonistic properties: Produces anti- 

 biotic matamycin, active upon gram-posit ive 

 bacteria. 



Habitat : Soil in Italy. 



29. Streptomyces bikiniensis Johnstone 

 and Waksman, 1948 (Johnstone, D. B. and 

 Waksman, S. A. J. Bacteriol. 55: 317-326, 



1948). 



Morphology: Sporophores straight. Spores 

 oval (Fig. 33). 



Sucrose nitrate agar: Growth white, be- 

 coming pallid neutral gray with white tinge. 

 Aerial mycelium abundant, white to gray. 

 Soluble pigment light brown. Superficial 

 droplets amber-colored. 



Glucose-asparagine agar: Growth abund- 

 ant. Aerial mycelium white to mouse-gray. 

 Soluble pigment light amber. 



Nutrient agar: Growth luxuriant. Aerial 

 mycelium moderate, white. Soluble pigment 

 deep brown. 



Starch agar: Growth abundant. Aerial 

 mycelium white, becoming gray. Slight hy- 

 drolysis. 



Potato: Growth wrinkled and raised, pale 

 ochraceous buff. Soluble pigment brown to 

 black. 



Gelatin: Slight liquefaction. 



Milk: Surface growth patchy, white. 

 Aerial mycelium gray. Gradual peptoniza- 

 tion. 



