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THE ACTINOMYCETES, Vol. II 



Aerial mycelium thin, powdery, white or 

 pale yellow to pale olive-buff. 



Nutrient agar: Growth white, later cream- 

 colored to light brown. Aerial mycelium 

 poor, white to gray. Soluble pigment brown. 



Milk: Growth in the form of ring on sur- 

 face, cream-colored to brown. Aerial myce- 

 lium white. Soluble pigment brown. Rapid 

 peptonization. 



Potato plug: Growth gray to olive-gray. 

 Aerial mycelium white. Usually no soluble 

 pigment. 



Nitrate reduction: Negative. 



Starch: Hydrolyzed. 



Carbon utilization: Utilizes glucose, fruc- 

 tose, galactose, or starch. Grows poorly on 

 sucrose, lactose, maltose, or inositol. Does 

 not utilize xylose, arabinose, raffinose, rham- 

 nose, mannitol, sorbitol, dulcitol, or salicin. 



Antagonistic properties: Produces an 

 antifungal agent designated as blastmycin. 



Remarks: Related to S. flavochromogenes. 



31. Streptomyces bobiliae (Waksman and 

 Curtis, 1916) Waksman and Henrici, 1948 

 (Waksman, S. A. and Curtis, R. E. Soil Sci. 

 1: 121, 1916; 8: 100, 1919). 



Morphology: Elongated sporophores form 

 a few close spirals of a dextrorse type. No 

 spirals according to Jensen (1930). Spores 

 oval and spherical. 



Sucrose nitrate agar: Growth abundant, 

 wrinkled, coral-red becoming deep red. 

 Aerial mycelium scant, white; later absent. 

 \'o soluble pigment. 



Glycerol malate agar: Growth cinnamon- 

 buff. No aerial mycelium. 



Glucose-asparagine agar: Growth coral- 

 red. No aerial mycelium. 



Nutrient agar: Growth gray, becoming 

 brownish to coral-red. No aerial mycelium. 

 Soluble brown pigment in presence of glyc- 

 erol (Jensen). 



Potato: Growth thin, dry, and wrinkled, 

 yellowish, becoming coral-red. No aerial my- 

 celium. Soluble pigment grayish to black. 



Gelatin: Growth cream-colored to orange. 



Aerial mycelium in the form of occasional 

 patches of white. Rapid liquefaction. Soluble 

 pigment brown. Melanin-positive. 



Milk: Dark brown ring. Peptonization 

 without coagulation. 



Starch media: Growth wrinkled, coral-red 

 with hyaline margin. Aerial mycelium white. 

 Hydrolysis medium. 



Nitrate: Good reduction to nitrite. 



Cellulose: No growth in solution. Good 

 growth on plate. 



Invertase: Posit ive. 



Production of H 2 S: Positive. 



Temperature: Optimum 37°C. 



Antagonistic properties: Produces pig- 

 mented antibiotic cinerubin. 



Habitat: Common in soil. 



Remarks: S. purpurascens is considered by 

 Corbaz et al. (1957) as a synonym of S. 

 bobiliae, except that the latter no longer 

 produces any aerial mycelium or spores. 

 Krassilnikov (1949) considered this species 

 as a variety of A. ruber. 



Type culture: IMRU 3310. 



32. Streptomyces bottropensis Konink. 

 Nederl. Gist et Spirit. (Konink. Nederland. 

 Gist et Spirit. Brit. Pat. 762,73(5, Dec. 5, 

 1956). 



Morphology: Aerial mycelium ramified, 

 with short, open spirals. Spores cylindrical, 

 elliptical to almost spherical, 1 to 4 by 0.6 

 to 1.2 m- 



Sucrose nitrate agar: Growth abundant, 

 reddish. Aerial mycelium limited. Soluble 

 pigment brown. 



Glucose-asparagine agar: Growth good, 

 yellow. Aerial mycelium limited, white to 

 pale gray. No soluble pigment. 



Calcium malate agar: Growth good, yel- 

 lowish-brown. Aerial mycelium white-gray. 

 No soluble pigment. 



Starch agar: Growth at first pink, later 

 darker (pH sensitive; acid-pink, alkaline- 

 blue). Aerial mycelium limited, while to 

 gray. Starch hydrolyzed. 



Glucose nutrient agar: Growth folded, 



