DESCRIPTION OF SPECIES OF STREPTOMYCES 



I s;> 



to :i limited extent, mannose, ^-inositol, 

 adonitol, lactose, ribose, raffinose, and cel- 

 lulose. 

 Type culture: [MRU 3798. 



:!7. Strt ptomyces caespitosus Sugawara and 

 Ilata, L956 (Sugawara, R. and Ilata, T. 

 J. Antibiotics (Japan) 9A: 1 17 L51, L956). 



Morphology: Primary verticils produced. 

 Spores oval, 1.3 to ().."> by ().:! to 0.5 u. 



Sucrose nitrate agar: Growth hyaline, 

 colorless to faint yellowish-brown. Aerial 

 mycelium white to yellowish-gray to green- 

 ish-yellow. Soluble pigmenl taint yellow. 



Calcium tnalate agar: Growth colorless 

 with yellow-brownish center, changing to 

 dark greenish-yellow to dull yellow. Aerial 

 mycelium white, with yellowish tinge, 

 greenish-yellow a1 the margin. Soluble pig- 

 menl taint yellow, pinkish shade in some 

 cultures. 



Glucose-peptone agar: Growth humid, 

 wrinkled, cracked in the center; colorless. 

 becoming greenish-yellow-gray, reddish- 

 gray, to dark gray. Aerial mycelium thin, 

 gray. Soluble pigment reddish-brown. 



Starch agar: Growth scanty, colorless to 

 taint yellowish-brown, or yellow to orange- 

 yellow. Aerial mycelium cottony, white, 

 cream with lavender patch in the center, 

 becoming yellowish-gray. Soluble pigmenl 

 taint yellowish-brown. 



Nutrient agar: Growth yellow-brown to 

 gray to dark gray. Aerial mycelium gray. 



Potato: Growth cream to brownish, center 

 Ligh.1 greenish-yellow. Aerial mycelium white 

 to grayish or gray with pale olive tinge. 

 Soluble pigmenl absent, or dark brown, or 

 grayish-brown. 



Milk: Surface ring yellow to pale yel- 

 lowish-brown. Xo aerial inyceliuni. Soluble 

 pigmenl pale brown. 



Gelatin: Growth cream-colored turning 

 greenish-yellow to reddish-yellow. Aerial 

 mycelium white to yellow. Soluble pigment 

 yellowish-brown. Rapid liquefaction. 



Tyrosinase reaction : None. 



Nitrate redud ion : Posil ive. 



Starch: Hydrolysis. 



Carbon utilization: Utilizes various car- 

 bohydrates; does not utilize xylose, rham- 

 nose, raffinose, arabinose, mannitol, salicin, 

 dulcitol, inulin, acetate, and succinate. 



Antagonistic properties: Produce- anti- 

 biotic mitomycin, active upon certain 

 neoplasms. 



Remarks: Closely related to S. kitasatoen- 

 sis and S. hachijoensis. 



38. Streptomyces caiusiae Dhala '/ <il.. 

 1957 (Dhala, S. A., Poonawalla, P. M., and 

 Bhatnagar, S. S. .1. Sci. & Ind. Res. L6C: 

 70 so, L957). 



Morphology: Aerial hyphae short and 

 straight; frequently clusters are produced, 

 subdividing a1 the distal portions into chains 

 of spores. Xo spirals formed either in syn- 

 thetic or nonsynthetic media; often tips of 

 the aerial hyphae slightly curved. Spore- 

 round to oval, 0.0 to 1.4 by 0.1 to 0.8 n- 



Sucrose nitrate agar: Colonies round. 

 convex, tough, with smooth surface when 

 unsporulated; citron-yellow, later turning 

 brown. Aerial mycelium white, turning yel- 

 lowish, then gray. Soluble pigmenl at first 

 yellow but later darkened to a brown tinge. 



Glucose-asparagine agar: Growth citron- 

 yellow. Aerial mycelium white. Soluble pig- 

 menl yellowish-brown. 



Xutrient agar: Growth wrinkled, with 

 irregular margins and radial ridges in old 

 cultures. White aerial mycelium. 



Starch agar: Colonies smooth, colorless 

 after 'J days; on further incubation, they 

 become large and wrinkled with radiating 

 ridges. Aerial mycelium white, turning gray. 

 Soluble pigmenl brown. Starch weakly hy- 

 drolyzed. 



Potato: Growth luxuriant, citron-yellow. 

 Aerial mycelium white, turning gray. Plug 

 turns black. 



Milk: Pellicle produced. Coloration of 

 milk brownish to black. Peptonization posi- 

 tive. 



