DESCRIPTION OF SPECIES OF STREPTOMYCES 187 



"with the exception of ATCC 3312, all Muk: Growth colorless to yellow. Coagu- 



isolates liquefy gelatin rapidly and produce lation and moderate peptonization. 



viomycin or similar antibiotic compounds." Cellulose: Growth yellow. No decomposi- 



Xii studies seem to have been made, how- i ion of cellulose. 



ever, of antibiotics produced by S. californi- Production of H2S: Negative. 



cms A.TCC 3312. Only because the original Carbon utilization: d-fructose, i-inositol, 



description of S. californicus gave spiral for- lactose, d-mannitol, rf-raffinose, ^-rhamnose, 



mation and these organisms did not, the sucrose, d-trehalose, and d(+)-xylose readily 



several viomycin-producing organisms and utilized; Z-arabinose, d-melibiose, and sali- 



the S. californicus A.TCC 3312 were de- cin utilized poorly; dextrin, esculin, deme- 



scribed as a variety of S. tins, us, namely lezitose, and adonitol no1 utilized at all. 



S. griseus var. purpureus. This was due to Antagonistic properties: Produces 



the fact thai S. griseus had the same mor- nucleocidin, an antibiotic possessing anti- 



phology and color of the aerial mycelium, trypanosomal properties. 



and because several streptomycin-producing Habitat: Soil in India. 



strains are known to form also a red-gray Remarks: This organism is closely related 



color in the substrate growth. to S. annulatus. 



Type culture: [MRU (ATCC) 3312. 41 streptomyces candidus (Krassilnikov, 



-10. Streptomyces minis Backus et al., MM 1) Waksman (Not Streptothrix Candida 



L957 (Backus, I':. .1., Tresner, II. I)., and Petruschky) . (Krassilnikov, X. A. Actino- 



Campbell, T. II. Antibiotics & Chemother- mycetales. Ezvest. Akad. Xauk. SSSR, 



apy 7: 532 541, 1957). Moskau, p. 4<), 1941). 



Morphology: Sporophores form short Morphology: Sporophores long, straigb.1 



loose spirals. Spores globose to elongated, or wavy, but never forming spirals; occa- 



0.6 to 1.0 by 1.0 to 1.8 n (Fig. 34). sionally arranged in broom-shaped bodies 



Sucrose nitrate agar: Growth cream-col- or fascicles. Spores oblong to cylindrical 



ored to yellowish. Aerial mycelium scanty, ("fragmentation spores"), '•() to 2.0 by 



white to gray. ().(> to 0.8 m- 



Glucose-asparagine agar: Growth ivory- Sucrose nitrate agar: Growth colorless. 



yellow. Aerial mycelium scanty, white. Aerial mycelium velvety, while. No soluble 



Calcium malate agar: Growth colorless to pigment. 

 yellow. Aerial mycelium scanty white to Nutrient agar: Growth good, lichenoid 

 gray. Crystalline pellets formed in growth or smooth. Aerial mycelium whitish. Mela- 

 zones, nin-negative. 



Starch agar: Growth colorless to yellowish. . Gelatin : Slow liquefaction. Melanin-nega- 

 Aerial mycelium white to mouse-gray. 



live. 



Potato: ( rrowth colorless, lichenoid. Aerial 

 mycelium poorly developed. X<> soluble pig- 

 ment or brownish, 

 soluble pigment . A elanin-neuat lve. Ain N ^ , .• , 



1 & Milk: No coagulation; good peptomza- 



Xutrient agar: Substrate growth lighl yel- 

 \\ . Aerial mycelium scanty, white. Nc 



tion. 



Starch: Rapid hydrolysis. 



Potato plug: Growth gray. Aerial myce 

 lium -canty, white io lighl gray. Plug dis- 

 colored. Cellulose: Good growth. 



Gelatin: Growth colorless to yellow. No Nitrate reduction : Positive. 



aerial mycelium. Partial liquefaction. No Sucrose: Inversion. 



soluble pigment. Production of IIS: Negative 



4, 



