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THE ACTIXOMYCETES, Vol. II 



straight; no spirals. Spores oval to elliptical; 

 surface smooth. 



Glycerol nitrate agar: Growth light yellow. 

 Aerial mycelium powdery, white. Soluble 

 pigment golden yellow. 



Glucose-asparagine agar: Growth smooth, 

 colorless to yellowish. Aerial mycelium 

 powdery, white. Soluble pigment faint yel- 

 low. 



Glycerol malate agar: Growth thin, 

 smooth, colorless to light yellow. Aerial my- 

 celium powdery, grayish-white. 



Nutrient agar: Growth poor, shiny, golden 

 yellow. Aerial mycelium white, powdery. 

 Soluble pigment golden yellow. Melanin- 

 negative. 



Glucose-peptone agar: Growth yellowish 

 with tinge of orange. Aerial mycelium gray- 

 ish-white. Soluble pigment light yellow to 

 golden yellow. 



Starch-casein agar: Growth colorless, with 

 yellowish reverse. Aerial mycelium powdery, 

 chalk-white. Strong hydrolysis of starch. No 

 soluble pigment . 



Potato: Growth heavy, yellow, becoming 

 brownish-yellow or orange. Aerial mycelium 

 cottony white to yellowish-white. 



Gelatin: Surface growth heavy, light to 

 dark yellow. Aerial mycelium white. Soluble 

 pigment yellow-brown to deep brown, only 

 in Liquefied portion. Strong liquefaction. 

 Melanin-negative. 



Milk: Growth colorless, with lighi yellow 

 reverse. Aerial mycelium cottony, snow- 

 white, becoming yellowish. Coagulation 

 slight. Strong peptonization. 



Cellulose: Growth very weak. 



Production of H 2 S: Negative. 



Antagonistic properties: Produces actino- 

 mycin C; some strains also produce the 

 antifungal cycloheximide. 



Habitat: Soil. 



Remarks: Ettlingerr/ al. (1958) considered 

 it as a member of the S. griseus group. A 

 complete description of this organism was 

 also given by Frommer (1058). Frommer 



(1959) described a variety of this organism 

 under the name fumigatus. It differed from 

 the type species by producing a mouse-gray 

 aerial mycelium on synthetic media, no 

 aerial mycelium on potato and gelatin, and 

 by displaying more limited proteolytic prop- 

 erties. 



Type culture: IMRU 3657. 



52. Streptomyces cinereoruber Corbaz et a/., 

 1957 (Corbaz, R., Ettlinger, L., Keller- 

 Schierlein, W., and Zahner, H. Arch. Mikro- 

 biol. 25:325-332, 1957). 



Morphology: Sporophores straight; no 

 spirals. Spores slightly elongated, 0.9 to 2 by 

 0.7 to lju; surface of spores smooth. 



Glycerol nitrate agar: Growth thin, light 

 carmine-red, in 7 days dark red. Aerial my- 

 celium ash-gray. Soluble pigment light car- 

 mine. 



Glucose-asparagine agar: Substrate 

 growth thin, greenish-gray to bluish-gray. 

 Aerial mycelium ash-gray. No soluble pig- 

 ment. 



Gelatin: Surface growth light carmine to 

 light brown. Aerial mycelium light gray. Sol- 

 uble pigment red-brown. Medium liquefac- 

 tion. Melanin-positive. 



Starch agar: Vegetative growth coral-red. 

 Aerial mycelium ash-gray. Soluble pigment 

 carmine. Hydrolysis limited. 



Potato: Growth lichenoid, brownish-yel- 

 low. Aerial mycelium ash-gray. Soluble pig- 

 ment bluish-gray. 



Milk: Pellicle light brown with sparse 

 aerial mycelium, powdery, white-gray. Coag- 

 ulation and peptonization. Reaction turns 

 acid. 



Carbon utilization: Utilizes xylose, arabi- 

 nose, and other sugars. Does not utilize l- 

 rhamnose, n-fructose, raffinose, inulin, <l- 

 sorbitol. 



Antagonistic properties: Produces anti- 

 biotic rhodomycin. 



Remarks: This organism is closely related 

 to S. bobiliae and S. purpurascens. S. cine- 

 reoruber var. fructofermentans is a variety, 



