200 



THE ACTIXOMYCETES, Vol. II 



Glucose-asparagine agar: Growth pale 

 green to yellowish-brown. Aerial mycelium 

 brownish-white to brownish-gray. Soluble 

 pigment light blue to yellowish-brown. 



Calcium malate agar: Growth light brown- 

 ish to brown. Aerial mycelium white to gray. 

 Soluble pigment greenish-blue to light 

 brown. 



Nutrient agar: Growth yellowish-brown to 

 brown. Aerial mycelium grayish-white. No 

 soluble pigment. 



Yeast extract agar: Growth pale yellow to 

 brown. Aerial mycelium light olive-gray. 

 Soluble pigment brown. 



Potato: Growth yellow to brown. Aerial 

 mycelium brownish-gray. Soluble pigment 

 dark brown. 



Gelatin: Growth yellow. Soluble pigment 

 yellow to brownish-yellow. Gelatin lique- 

 fied. 



Milk: Produces a sedimented growth with- 

 onl any soluble pigment. Milk coagulated 

 but not peptonized. 



Carbon utilization: Utilizes various sugars 

 and salts of organic acids, but not xylose, 

 acetate, or citrate. 



Antagonistic properties: Produces blue 

 antibiotic cyanomycin, active against gram- 

 positive and gram-negative bacteria; also 

 produces aureothricin-like substances. 



64. Streptomyces cylindrosporus (Krassil- 

 nikov, L941) Waksman (Krassilnikov, N. A. 



Actinomycetales. Izvest. Akad. Nauk. SSSR, 

 Moskau, p. 57, 1941). 



Morphology: Sporophores straight, 

 branched. Spores cylindrical or oblong, 1.0 

 to 1.7 by 0.7 ij.. 



Sucrose nitrate agar: Colonies velvety, 

 dark brown or chocolate. Aerial mycelium 

 white-gray to brown-gray. Soluble pigment 

 brown. 



\ ut lient agar: ( rrowth dark brown. Aerial 

 mycelium white. Soluble pigment brown. 



Glucose-asparagine agar: Growth brown. 

 Aerial mycelium white-gray. No soluble pig- 

 ment. 



Gelatin: Weak Liquefaction. Melanin-posi- 

 tive. 



Milk: Coagulation limited, peptonization 

 weak; color of milk brown to almost black. 



Potato: Substrate growth brown. Aerial 

 mycelium light gray. Soluble pigment brown. 



Starch: Weak hydrolysis. 



Cellulose: Limited, colorless growth. Aer- 

 ial mycelium white. 



Nitrate reduction: Positive. 



Sucrose inversion: Negative. 



Antagonistic properties: None. 



Habitat : Soil. 



Remarks: The description of the organism 

 has been supplemented by Hoffmann (1958). 

 It appears to be related to S. vinaceus, S. 

 purpureochromogenes, and S. purpeoftt.se us. 

 ( rause et al. (1957) described a related form 

 as A. umbrinus. 



Type culture: IMRU 3764. 



(So. Streptomyces diastaticus (Krainsky) 

 Waksman and Henrici (Krainsky, A. Centr. 

 Bakteriol. Parasitenk. Abt. II, 41: 682, 

 1914). 



Morphology: Sporophores form tight spi- 

 rals. Spores oval or spherical, 1.0 to 1.2 by 

 1.1 to 1.5 ii (Figs. 37, 38). 



Sucrose nitrate agar: Growth thin, gray. 

 Aerial mycelium white, becoming drab gray. 



Calcium malate agar: Colonies 2 to 4 mm, 

 yellowish when old. Aerial mycelium gray, 

 with white outer zone; white specks fre- 

 quently produced in gray mycelium. 



Glucose-asparagine agar: Growth yellow- 

 ish, spreading. No aerial mycelium. 



Nutrient agar: Growth cream-colored. 

 Aerial mycelium white, then gray. Soluble 

 brown pigment. 



Potato: Growth white-gray. Aerial myce- 

 lium gray and white. 



Gelatin: Liquefaction, with small, cream- 

 colored flakes in liquefied part. 



Milk: Brownish ring. Coagulation and 

 slow peptonization. 



Starch agar: Growth thin, colorless. 



