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THP] ACTINOMYCETES, Vol. II 



84. Streptomyces flavoreticuli Funaki et al., 

 1958 (Funaki, M., Tsuchiya, F., Maeda, 

 K., and Kamiya, T. J. Antibiotics (Japan) 

 11A: 138-142, 1958). 



Morphology: Aerial mycelium is long with 

 many short branches; numerous small 

 verticils are produced depending on the 

 nature of the medium, especially on starch- 

 ammonium agar. 



Sucrose nitrate agar: Growth colorless to 

 pale yellow. Aerial mycelium white, cottony. 

 Soluble pigment faint yellow. 



Glucose-asparagine agar: Growth pale 

 yellow to yellowish-brown. Aerial mycelium 

 yellowish-white to olive-gray. Soluble pig- 

 ment pale yellow. 



Calcium malate agar: Growth pale yellow. 

 Aerial mycelium pale yellow. Xo soluble 

 pigment. 



Nutrient agar: Growth yellow to brown. 

 Aerial mycelium pale yellow to yellowish- 

 gray. Soluble pigment brown. 



Potato: Growth yellow, folded. Aerial 

 mycelium yellowish white to olive-gray or 

 olive-yellow. Soluble pigment dark brown. 



Milk: Colorless pellicle. Aerial mycelium 

 white. Soluble pigment brown. Milk coagu- 

 lated, then peptonized. 



Gelatin: Growth yellowish-brown. Aerial 

 mycelium white to light gray. Soluble pig- 

 ment brown. Gelatin liquefied. 



Starch: Not hydrolyzed. 



Antagonistic properties: Produces anti- 

 biotic virocidin, which possesses antiviral and 

 antibacterial properties. 



Remarks: Similar to S. reticuli and to S. 

 Jim- us, differing from the first by the forma- 

 tion of yellow growth and yellow soluble 

 pigment and from the second by the forma- 

 tion of verticils. 



85. Streptomyces flavovirens (Waksman, 

 191'.)) Waksman and Henrici, 1948 (Waks- 

 man, S. A. Soil Sei. 8: 117, 1919). 



Morphology: Sporophores coarse, straight, 

 and short, relatively unbranched; large 

 masses of minute tufts; open spirals may be 



produced in certain substrates. Spores 

 spherical, oval to rod-shaped, 0.75 to 1.0 by 

 1 .0 to 1 .5 M . 



Sucrose nitrate agar: Growth yellowish 

 with greenish tinge. Aerial mycelium gray. 

 Soluble pigment greenish-yellow. 



Glucose-asparagine agar: Growth re- 

 stricted, developing only to a very small 

 extent into the medium, yellow, turning 

 black. Soluble pigment golden yellow to 

 greenish-yellow. 



Nutrient agar: Growth yellowish; reverse 

 dark in center with yellowish zone and 

 outer white zone. 



Potato: Growth sulfur-yellow, wrinkled. 



Gelatin: Surface pellicle yellowish-green. 

 Good liquet action. Melanin-negative. 



Milk: Cream-colored to brownish ring; 

 coagulation and peptonization. 



Starch agar: Growth greenish-yellow, 

 spreading, developing deep into the medium. 

 Good hydrolysis. 



Invertase: Negative. 



Nitrate reduction: Limited. 



Production of H- 2 S: Negative. 



Cellulose: No growth. 



Antagonistic properties: Produces actino- 

 mycin. 



Habitat : Soil. 



Remarks: Certain forms belonging to this 

 species, such as A. griseostramineus and A. 

 olivaceoviridis, have been described by Gause 

 et al. (1957). Ettlinger et al. (1958) considers 

 this species as belonging to the S. fradiae 

 group. Hirsch (1960) considers this organism 

 as an oligonitrophilic form. 



Type culture: IMRU 3320. 



8(>. Streptomyces flavus (Krainsky, 1914) 

 Waksman and Henrici, 1948 (Krainsky, A. 

 Centr. Bakteriol. Parasitenk. Abt. II, 41: 

 (585, 1914; Waksman, S. A. and Curtis, 

 R. E. Soil Sci. L: 99, 1916; 8: 71, 1919). 



Not .1. flavus Krainsky emend. Krassil- 

 nikov (1941). 



Morphology: Sporophores are long, usn- 



