DESCRIPTION OF SPECIES OF STREPTOMYCES 



211 



ally no spirals; some open spirals may be 

 produced. Spores oval, 1 .2 m- 



Sucrose nitrate agar: Growth yellow or 

 sulfur-yellow. Aerial mycelium straw-yellow. 



Glucose-asparagine agar: Growth sulfur- 

 yellow, center shading to brown. Aerial 

 mycelium white to gray. 



Nutrient agar: Growth gray, spreading, 

 folded. Aerial mycelium white, appears late. 



Starch agar: Growth cream-colored with 

 pink tinge. Hydrolysis marked. 



Potato: Growth yellow. Aerial mycelium 

 gray. Melanin-negative. 



Gelatin: Growth in form of small, yellow- 

 ish masses on surface. Rapid liquefaction. 

 Melanin-negative. 



Milk: Rapid coagulation and peptoniza- 

 tion. 



Sucrose inversion: Negative. 



Nitrate: No reduction. 



Cellulose: ( Irowth poor. 



Temperature: Optimum 25°C. 



Antagonistic properties: Some strains 

 produce actinomycin and certain other 

 antibiotics. 



Habitat : Soil. 



Remarks: Represents a large group of 

 species, as shown previously (Chapter 3). 

 Above description is Rased largely upon that 

 given by Krainsky. According to Ettlinger 

 ei al. (1958), this organism does not form 

 any spirals (as found also by Waksman and 

 Curtis) and is related to S. olivaceus. 



Type culture: [MRU 3321. 



87. Streptomyces flocculus (Duche, L934) 

 Waksman and Henrici ( Duche, J. Res 

 actinomyces du groupe albus. R. Lechevalier, 

 Paris, L934). 



Sucrose nitrate agar: Growth cream- 

 colored, later covered with white aerial 

 mycelium. No soluble pigment. 



Glucose-asparagine agar: Growth limited. 

 cream-colored, only slightly raised above the 

 surface of the medium; occasionally abun- 

 dant growth produced with white aerial 

 mycelium, colorless on reverse side. 



Nutrient agar: Growth cream-colored, 



later covered with white aerial mycelium. 

 No soluble pigment . 



Rotato: Growth punctiform. Aerial myce- 

 lium white. Soluble pigment faint, yellowish. 



Gelatin: Growth limited. Liquefaction 

 slow-. Melanin-negative. 



Milk: Growth rose-colored. Peptonization 

 slow. 



Coagulated serum: Growth cream-colored. 

 Aerial mycelium line, white. Liquefaction 

 slow. 



Tyrosine medium: Growth whitish. No 

 soluble pigment. 



Production of HjS: Negative. 



Remarks: Belongs to the S. albus series. 



88. Streptomyces fradiae (Waksman and 

 Curtis, R)l(i) Waksman and Henrici, L948 

 (Waksman, S. A. and Curtis, R. E. Soil Sci. 

 1: 99-134, 1916; 8: 1)0, 1919). 



Morphology: Sporophores branched 

 monopodially, straight or flexible, but no 

 true spirals. On certain media, such as 

 glycerol agar, spirals are formed. Ettlinger 

 et al. (1958) found open spirals. Spores oval 

 to rod-shaped, 0.5 by 0.7 to L.25 m. smooth 

 (Fig. 39). 



Sucrose nitrate agar: Growth smooth, 

 spreading, colorless, or pale yellow-orange. 

 Aerial mycelium thick, cottony, seashell- 

 pink. No soluble pigment . 



Malate-glycerol agar: Growth orange. 

 Aerial mycelium seashell-pink. 



Glucose-asparagine agar: Growth re- 

 stricted, glossy, buff-colored, lichenoid mar- 

 gin. Aerial mycelium appears late, seashell- 

 pink. 



Nutrient agar: Growth restricted, yellow- 

 ish, becoming orange-yellow to buff. No 

 aerial mycelium. No soluble pigment. 



Potato: Growth restricted, orange-colored. 

 Aerial mycelium white to rose or pink. Solu- 

 ble pigment absent or faint brown. 



Gelatin: Growth dense, cream-colored to 

 brownish. Aerial mycelium white. Gelatin 

 liquefied. No soluble pigment. 



