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THK ACTINOMYCETES, Vol. II 



II. C, and Harris, I). A. J. Bacterid. 56: 

 259, 1948; Waksman, S. A. Proc. Natl. 

 Acad. Sci. U. S. 45: 1043-1047, 1959). 



Synonyms: Actinomyces globisporus Kras- 

 silnikov, 1941. Actinomyces globisporus sub- 

 sp. streptomycini (Waksman) Krassilnikov, 

 1949. Actinomyces streptomycini Krassilni- 

 kov, 1957. 



Morphology: Sporophores straight, pro- 

 duced in tufts (Fig. 41). Spores spherical to 

 oval, 0.8 by 0.8 to 1.7 m; surface smooth (PI. 



iii). 



Sucrose nitrate agar: Growth thin, spread- 

 ing, colorless, becoming olive-buff. Aerial 

 mycelium thick, powdery, water-green. Pig- 

 ment insoluble. 



Nutrient agar: Growth abundant, almost 

 transparent, cream-colored. Aerial mycelium 

 powdery, white to light gray. No soluble 

 pigment. 



Glucose agar: Growth elevated in center, 

 radiate, cream-colored to orange, erose mar- 

 gin. 



Figure 41. Substrate and aerial mycelium of 

 S. grist us. 



Starch media: Growth thin, spreading, 

 transparent. Hydrolysis strong. 



Tyrosine agar: Dark pigment often pro- 

 duced. 



Potato: Growth wrinkled, yellowish to 

 brownish, covered with white, powdery aer- 

 ial mycelium. 



Gelatin: Greenish-yellow or cream-colored 

 surface growth with brownish tinge. Rapid 

 liquefaction. 



Milk: Cream-colored ring; coagulation 

 with rapid peptonization, becoming alkaline. 



Cellulose: Scant to fair growth. 



Nitrate reduction: Positive. 



Pigments: Produces green or yellow solu- 

 ble pigment on calcium malate and succinate 

 media. 



Production of H 2 S: Negative. 



Carbon sources: See S. griseinus. 



Antagonistic properties: Strongly antago- 

 nistic. Produces antibiotic streptomycin, ac- 

 tive against a large number of bacteria and 

 actinomycetes, but not against most fungi or 

 viruses; also produces cycloheximide, active 

 upon fungi. Resistant to streptomycin-pro- 

 ducing organisms and to streptomycin. 



Remarks: An extensive literature has ac- 

 cumulated on the nature of this organism 

 (Koreniako and Nikitina, 1959), on its phage 

 sensitivity (Koerber et al., 1950), antibiotic 

 production (Waksman, 1949), etc. 



Habitat: Soils, river muds, throat of 

 chicken. 



Type culture: [MRU 3463. 



117. Streptomyces hachijoensis Vamaguchi, 

 1954 (Yamaguchi, T. J. Antibiotics (Japan) 

 7 A: 10-14, 1954). 



Morphology: Aerial hyphae short , straight, 

 0.6 to 1.2 m- Secondary verticils produced. 

 Spores cylindrical, 0.8 to 1.0 by 1.5 to 1.8 m- 



Sucrose nitrate agar: Growth restricted, 

 colorless; reverse yellowish. Aerial mycelium 

 white, changing to pinkish-buff. No soluble 

 pigment. 



Calcium malate agar: Growth colorless to 



