DESCRIPTION OF SPECIES OF STREPTOMYCES 



227 



yellow. Aerial mycelium white to pale pink- 

 ish-buff. Soluble pigmenl absent. 



Nutrienl agar: Growth cream-colored, 

 wrinkled. Aerial mycelium powdery, shade of 

 pale ochraceous-buff. No soluble pigment. 



Potato: Growth cream-colored to yellow- 

 ish, wrinkled, raised. Aerial mycelium white. 

 Soluble pigment around growth faint pur- 

 plish. 



Blood agar: Growth yellow to brownish- 

 yellow. Aerial mycelium flocculent, white. 

 Soluble pigment dark. Positive hemolysis. 



Gelatin: Growth yellow to brown. No 

 aerial mycelium. Rapid liquefaction. 



Milk: Surface ring yellow to brown. Aerial 

 mycelium in form of white patches. Soluble 

 pigment pinkish to orange. Coagulation fol- 

 lowed by peptonization. 



Nitrate reduction: Negative. 



( 'ellulose: No growth. 



Antagonistic properties: Produces an anti- 

 fungal agent, trichomycin, a member of the 



candicidin group of antibiotics. 



Remarks: Resembles S. rubrireticuli. Bli- 

 nov (1958) described a variety (fuscatus) of 

 this species, as a producer of candicidin- 

 type antibiotics. 



118. Streptomyces halstedii (Waksman and 

 Curtis, L916) Waksman and Henrici, 1948 



(Waksman, S. A. and Curtis, R. K. Soil Sci. 

 1: 124, 1916;8: 121, 1919). 



Morphology: Sporophores form closed 

 spirals. Spores oval or rod-shaped, 1.0 to 

 1.2 by 1.2 to 1.8 /x- 



Sucrose nitrate agar: Substrate growth 

 abundant, spreading, raised, at first light 

 colored, becoming dark to almost black. 

 Aerial mycelium white, turning dull gray. 

 No soluble pigment. 



( rlycerol malate agar: ( trowth dark. Aerial 

 mycelium deep mouse-gray. 



Nutrient agar: Growth restricted, wrin- 

 kled, cream-colored. No aerial mycelium. 

 Melanin-negat ive. 



Glucose-asparagine agar: Growth wrin- 



kled, center elevated, edge lichenoid, color- 

 less, becoming brown. No aerial mycelium. 



Potato: Growth abundant, moist, wrin- 

 kled, cream-colored with green tinge. 



Gelatin: Small, cream-colored masses of 

 growth in bottom of tube. Rapid liquefac- 

 tion. No soluble pigment . 



Milk: Cream-colored ring. Coagulation 

 and slow peptonization. 



Starch media: Growth abundant, glossy, 

 brownish. No aerial mycelium. Rapid hy- 

 drolysis. 



( ellulose: No growth. 



Nitrate: Reduction to nitrite. 



Production of IBS: Negative. 



Temperature: Optimum :-)7°C. 



Antagonistic properties: Strongly antag- 

 onistic; some strains show only antifungal 

 activity; some strains produce carbomycin. 



Habitat: Soil. 



Remarks: Several closely related forms 

 have been described. According to Ettlinger 

 et al. (1958), the strains examined produce 

 no spirals and belong to S. olivaa us. Ac- 

 cording to Okami and Suzuki (1958), the 

 sporophores are wavy, seldom forming hooks 

 or primitive spirals. Gause et al. (1957) de- 

 scribed a closely related form as .1. griseoirtr 

 car not us. 



Type culture: [MRU 3328. 



IP.). Streptomyces hawaiiensis Cron et el., 

 1956 (Cron, M. J., Whitehead, D. F., Hooper, 

 B R., Heinemann, B., and Lein, .1. Anti- 

 biotics & Chemotherapy 6: (i:; 67, 1956). 



Morphology: Sporophores produce spirals 

 on some media. Spores oval, 0.6 to 0.8 by 

 0.7 to 1.3/*. 



Sucrose nitrate agar: ( rrowth faint yellow. 

 Aerial mycelium sparse, while to flesh-col- 

 ored. Soluble pigmenl faint tan or absent. 



Glucose-asparagine agar: Growth light 

 brownish. Aerial mycelium moderate, white 

 to gray. Soluble pigment faint tan or absent . 



Nutrient agar: Growth gray with light 

 brown reverse. Aerial mycelium limited. 



