2:!2 



Till; ACTINOMYCOTIC, Vol. II 



Remarks: Above description was supple- 

 mented by Hoffmann (1958). Krassilnikov 

 ( L949) considers this organism as a variety of 

 .1 . cretaceus. 



Type culture: IMRU 3329. 



L26. Streptomyces ipomoeae (Person and 

 Martin, 1940) Waksman and Henrici, 1948 

 (Person, L. H. and Martin, W. J. Phyto- 

 pathology 30: 913, 1940). 



Morphology: Spores oval to elliptical, 0.9 

 to L.3 by L.3 to 1.8 y.. 



Sucrose nitrate agar: Growth abundant, 

 wrinkled, olive-yellow. Xo aerial mycelium. 



Xiitrieni agar: Growth moderate, in form 

 of small, shiny colonies, both on the surface 

 and imbedded in the medium, silver-colored. 



Starch agar: Growth moderate, smooth, 

 ivory-colored. Aerial mycelium white with 

 patches of bluish-green. Xo soluble pigment. 

 Complete hydrolysis after 12 days. 



Potato: Growth moderate, shiny, wrin- 

 kled, light brown. No aerial mycelium. No 

 soluble pigment. 



Gelatin: Growth scanty, after 25 days at 

 20°C. No aerial mycelium. No soluble pig- 

 ment. Some liquefaction. 



Milk: Ring on surface. No visible coagu- 

 lation; positive peptonization. 



C ellulose: Xo growth. 



Nitrate reduction: Positive. 



Antagonistic properties: Positive. 



Habitat: Lesions caused by sweet-potato 

 disease. 



Type culture: [MRU 3476. 



127. Streptomyces kanamyceticus Okami 

 and Umezawa, 1957 (Umezawa, H., Ueda, 

 M., Maeda, K., Yagishita, Iv., Kondo, S., 

 Okami, Y., Utahara, R., Osato, Y., Nitta, 

 K., and Takeuchi, T. J. Antibiotics (Japan) 

 I0A: 181-188, 1957). 



Morphology: Sporophores flexible and 

 hooked, no true spirals. 



Glycerol nitrate agar: Growth at first 

 colorless, later lemon-yellow. Aerial myce- 

 lium white to yellow, occasionally with a 



greenish or faint pinkish tinge. Soluble pig- 

 ment occasionally produced, faint brown. 



Glucose-asparagine agar: Growth color- 

 less to yellow with faint pinkish-white, and 

 yellow or hay-colored reverse. Aerial myce- 

 lium scant; develops from center of colony, 

 white to faint pinkish-white to greenish- 

 yellow or yellow. Soluble pigment occasion- 

 ally produced, faint brown. 



Calcium malate agar: Growth yellow. 

 Aerial mycelium white-yellow. 



Nutrient agar: Growth cream-colored. 

 Aerial mycelium absent or white. Xo soluble 

 pigment. 



Potato: Growth wrinkled, faint yellowish- 

 brown to yellow. Aerial mycelium scant, 

 white. Xo soluble pigment. Substrate be- 

 neath growth occasionally changes to brown. 



Gelatin: Liquefaction positive. Xo soluble 

 pigment. Melanin-negative. 



Milk: Growth colorless. Aerial mycelium 

 absent or white. Coagulation and peptoni- 

 zation doubtful. 



Blood agar: Growth wrinkled, grayish- 

 reddish-brown. Xo aerial mycelium. Xo sol- 

 uble pigment. 



Starch: Hydrolyzed. 



Carbon utilization: Utilizes arabinose, 

 dextrin, fructose, galactose, glycerol, mal- 

 tose, mannitol, mannose, raffinose, starch, 

 sucrose, and succinate. Does not utilize ino- 

 sitol, inulin, lactose, rhamnose, sorbose, xy- 

 lose, and acetate. Some strains grow slightly 

 on esculin, salicin, sorbitol, and citrate. 



Antagonistic properties: Produces kana- 

 mycin, an antibiotic related to the neomycin 

 group. 



Remarks: Closely related to S. albido- 

 flavus, S. lieskei, and S. alboflavus. Okami 

 et al. (1959a) made a detailed study of the 

 mutants produced by this organism. 



128. Streptomyces kentuckensis Parr and 

 Carman, 1956 (Han-, V. S., and Carman, 

 P. E. Antibiotics & Chemotherapy 6: 286 

 289, 1950). 



Morphology: Aerial mycelium thick- 



