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THE ACTINOMYCETES, Vol. II 



dinskaia, L. K., and Vesselov, N. M. Mikro- 

 biologiya 26: 558-564, 1957). 



Morphology: Substrate mycelium mono- 

 podially branched, 0.7 to 0.8 n in diameter. 

 Sporophores produce spirals with 1 to 3 

 turns. Spores spherical, oval, seldom elon- 

 gated. Sporulation is generally weak, oc- 

 curring only on certain media; spiral forma- 

 tion occurs seldom, largely on synthetic 

 media with a limited amount of sucrose, and 

 on starch media. 



Sucrose nitrate agar: Growth yellow- 

 orange. Aerial mycelium white with rose 

 tinge. 



Nutrient agar: Substrate growth colorless, 

 free of aerial mycelium. No soluble pigment. 

 In old cultures, clumps of aerial hyphae may 

 be formed. 



Potato agar: Growth yellow-orange. Aerial 

 mycelium white with rose tinge. Crystals of 

 salts deposited along the mycelium in the 

 substrate. 



Milk: Coagulation weak; rapid peptoniza- 

 tion. 



Gelatin: Not liquefied in 10 days. 



Starch: Moderate hydrolysis. 



Nitrate: Reduced. 



Sucrose: Not inverted. 



Cellulose: No growth. 



Carbon utilization with acid formation: 

 arabinose, inositol, sorbitol; no acid from 

 glucose, lactose, rhamnose, xylose, inulin, 

 inositol, mannitol, or dulcitol. 



Antagonistic properties: Produces anti- 

 bacterial and antiviral (luridin) substances. 



Remarks: Cannot be distinguished from 

 S. fradiae in its morphological and cultural 

 properties, but is different in its biochemical 

 and its antibiotic activities. 



141. Streptomyces luteoverticillatus Shin- 

 obu, 1956 (Shinobu, R. Mem. Osaka Univ. 

 B (N.S.) 5: 84 93, L956). 



Morphology: Primary and secondary 

 verticils produced on various synthetic 

 media. Spores coccoid to elliptical, about 0.8 

 M long. 



Sucrose nitrate agar: Growth pale brown. 

 Aerial mycelium cottony, brownish-white 

 to brown. 



Glucose-asparagine agar: Growth thin, 

 brown. Aerial mycelium cottony, yellow- 

 white. Soluble pigment pale brown. 



Nutrient agar: Growth excellent, deep 

 brown. Aerial mycelium white to yellow to 

 green. Soluble pigment deep brown. 



Potato plug: Growth heavy, brown. Aerial 

 mycelium yellow to greenish. Soluble pig- 

 ment brown. 



Milk: Aerial mycelium heavy, brown. 

 Coagulation uncertain; peptonization strong. 

 Soluble pigment brown. 



Gelatin: Strong liquefaction. 



Diastase reaction: Strong. 



Tyrosinase reaction: Strong. 



Carlton utilization: fructose, mannitol, 

 and inositol utilized. Xylose, rhamnose, 

 sucrose, lactose, and raffinose not utilized. 



Habitat: Soil. 



Remarks: Resembles S. verticillatus. 



142. Streptomyces lydicus DeBoer et al., 

 L955 (DeBoer, G, Dietz, A., Silver, W. S., 

 and Savage, G. M. Antibiotics Ann. 1955- 

 L956, p. 886-892). 



Morphology: Sporophores long, slightly 

 coiled at tip. Spores spherical to oval. 



Sucrose nitrate agar: Substrate growth 

 buff-colored. Aerial mycelium white. 



Nutrient agar: Some substrate growth. 

 No aerial mycelium. Soluble pigment yellow- 

 ish. 



Casein-yeast extract -beef agar: Growth 

 olive-tan. Aerial mycelium gray-white with 

 flecks of black. Soluble pigment olive-tan. 



Glucose-peptone agar: Aerial mycelium 

 gray-white. Soluble pigment yellow. 



Starch agar: Growth good. Aerial myce- 

 lium pink-gray-white. Hydrolysis good to 

 excellent. 



Gelatin: Some growth. No aerial myce- 

 lium. Liquefaction positive. Soluble pigment 

 olive-colored. 



