DESCRIPTION OF SPECIES <>K STREPTOMYCES 



■2:\\) 



Milk: Ring around surface. Peptonization 

 positive. 



Nitrate reduction: Positive. 



Carbon utilization: Most sugars and 

 organic acids utilized, hut not rhamnose, 

 inulin, dulcitol, inositol, or the sodium salts 

 of formic, oxalic, tartaric, and salicylic acids. 



Production of H 2 S: Negative. 



Antagonistic properties: Produces an anti- 

 biotic, streptolydigin, active against various 

 bacteria. 



Remarks: Related to S. diastaticus. 



143. Streptomyces macrosporeus Ettlinger 

 et al, 1958 (Ettlinger, L., Corbaz, R., and 

 Hiitter, R. Arch. Mikrobiol. 31: 346, 1958). 



Morphology: Sporophores monopodially 

 branched along the whole axis with open, 

 irregular spirals. Spores large, 1.7 to 2 by 

 1 .5 to 2 /x; short spines (Plate II m). 



Glycerol nitrate agar: Substrate growth 

 yellow. Aerial mycelium white-yellow to ash- 

 gray. Soluble pigment golden yellow. 



Glucose-asparagine agar: Growth milk- 

 white. No aerial mycelium. Soluble pigment 

 whitish-yellow. 



Calcium malate agar: Growth yellow. 

 Aerial mycelium white-yellow to ash-gray. 

 Soluble pigment white-yellow. 



Starch agar: Growth light yellow. Aerial 

 mycelium white-gray. Good hydrolysis of 

 starch. 



Potato: Growth abundant, light yellow to 

 golden yellow. Aerial mycelium ash-gray. 



Gelatin: Growth sparse. Liquefaction 

 slow. Xo soluble pigment. 



Milk: Pellicle light yellow to yellow- 

 brown. Aerial mycelium white to white-gray. 

 Coagulation strong; no peptonization. 



Antagonistic properties: Produces an 

 antibiotic, carbomycin. 



Habitat : Soil in Madras, India. 



144. Streptomyces maculatus (Millard and 

 Burr, 1926) Waksman and Henrici, I'.MN 

 (MiUard, W. A. and Burr, S. Ann. Appl. Biol. 

 13: 580, 1926). 



Morphology: Growth tough, shiny, carti- 

 laginous. Aerial mycelium rarely produced, 

 though in certain strains it may frequently 

 occur, especially when grown on organic 

 media. Sporophores straight, short. Spores 

 spherical, 0.5 to 0.6 /x- 



Sucrose nitrate agar: Growth orange- 

 yellow to orange-red; as the culture ages it 

 may change to dark green or black. Xo 

 aerial mycelium. 



Nutrient potato agar: Growth vinaceous- 

 tawny. Soluble pigment vinaceous-tawny. 



Potato: Growth restricted, raised, pinkish. 

 Aerial mycelium scant, white. Soluble pig- 

 ment gray to brown. 



Gelatin: Growth limited. Liquefaction 

 slow. 



Milk: Growth slight. Xo coagulation; no 

 peptonization. 



Starch : Hydrolyzed. 



Nitrate reduction: Negative. 



Tyrosinase reaction: Negative. 



Oxygen requirement: Said to grow well 

 under anaerobic conditions. 



Paraffin: Not utilized. 



Temperature: Grows well at 37.5°C. 



Habitat: Potato scab and soil. 



Type culture: IMRU 3376. 



145. Streptomyces madurae (Vincent, 

 1894) now comb. (Vincent, H. Ann. inst. 

 Pasteur 8: 129, 1894). 



Synonyms: N. babiensis, N. brumpti, X. 

 madurae, and A', salmonicolor (A. salmoni- 

 color Millard and Burr, 1926). Baldacci (1944) 

 listed 17 synonyms. 



Strains of this organism were reported by 

 various investigators, most recently by 

 Gonzalez Ochoa and Sandoval (1951), to 

 form, under certain conditions of culture 

 and on certain media, such as grain, an 

 aerial mycelium, with slraight or spiral- 

 shaped sporophores; the spores were cylindri- 

 cal or oval. This led them to consider this 

 organism as a Streptomyces. Mariat (1957) 

 was also of the same opinion. MacKinnon 

 and Artagaveytia-Allende (1956) consider 



