240 



THE ACTIXOMYCETES. Vol. II 



the generic position of this species as far 

 from settled. 



Morphology: Growth red to red-brown or 

 pink. In tissues it forms grannies consisting 

 of radiating actinomycosis. Initial branched 

 mycelium is said to be nonsegmented. Not 

 acid-fast. Aerial mycelium white and pink 

 in color. 



Glucose-asparagine agar: Growth cream- 

 colored. Some cultures give reddish pig- 

 mentation. 



Protein media: Growth good, pinkish. 

 Soluble pigment brown. 



Gelatin: Growth glistening, at first white, 

 then buff to rose or crimson. Soluble pigment 

 irregular and unpredictable, occasionally red. 

 Gelatin slowly liquefied. 



Milk: No change, or slight; coagulation 

 slow, if any; peptonization slow. 



Carbon utilization: Utilizes starch, glu- 

 cose, mannitol, and xylose, but not lactose 

 or paraffin. 



Nitrate reduction: Positive. 



Pathogenicity: This property was vari- 

 ously reported. Topley and Wilson (1946) 

 stated that this organism causes a local 

 tissue disease when inoculated under the skin 

 in guinea pigs. Often reported as not patho- 

 genic for the usual laboratory animals; 

 pathogenic for monkeys. 



Source: Wide geographical distribution. 

 Madura foot and other substrates. 



146. Streptomyces marginatus (Millard and 

 Burr, 1926) Waksman (Millard, W. A. and 

 Burr, S. Ann. Appl. Biol. 13: 580, 11)20). 



Morphology: Sporophores straight. Spores 

 oval to spherical, 0.0 by 0.8 fi. 



Sucrose nitrate agar: Growth thin, echi- 

 nate. Aerial mycelium olive-buff. Soluble 

 pigment cream-colored. 



Nutrient potato agar: Growth heavy, 

 gray. Aerial mycelium white to whitish- 

 yellow. Soluble pigment light golden brown 

 to deep golden brown. 



Potato: Growth good, raised. Aerial myce- 



lium abundant, buff to olive-buff. Plug at 

 first gray, later becoming black. 



Gelatin: Growth thin, pale olive-gray. 

 Aerial mycelium abundant, pale gray to 

 olive-buff. Soluble pigmenl buff. Liquefac- 

 tion rapid. 



Milk: Growth good. Aerial mycelium 

 white. Coagulation and peptonization. 



Starch: Hydrolysis. 



Nitrate reduction: Positive. 



Temperature: Grows well at 37.5°C. 



Habitat: Potato scab. 



147. Streptomyces marinolimosus (Zobell 

 and Upham, 1044) Waksman (ZoBell, C. E. 

 and Upham, H. G. Bull. Scripps Inst. 

 Oceanogr. Univ. Calif. 5: 230-202, 1044). 



Morphology: Aerial mycelium consists of 

 branching filaments with chains of spores. 

 Spores 0.0 m in diameter. 



Agar media: Growth dull. Aerial myce- 

 lium white to pinkish, powdery, rough, in 

 concentric circles. Odor of freshly turned 

 soil. 



Potato: Growth yellow, becoming white, 

 powdery, raised, rugose. Potato darkened. 



Gelatin: Growth in form of flat, yellowish, 

 circular colonies, with tendency to grow 

 together. Liquefaction crateriform. Melanin- 

 negative. 



Sea water broth: Growth in form of light 

 yellow clumps; pellicle produced on surface. 

 Earthy odor. 



Milk: Growth in form of pellicle. Com- 

 plete peptonization in 20 days. 



Starch: Hydrolysis. 



Nitrate reduction: Positive. 



Production of H 2 S: Positive. 



Source: Marine mud. 



Remarks: All differential media were pre- 

 pared with sea water. 



148. Streptomyces marinus (Humra and 

 Shepard, 1946) nov. com)). (Humm, H. J. 

 and Shepard, K. S. Duke Univ. Marine Sta. 

 Bull. 3: 77, 1046). 



Morphology: Sporophores sometimes form 



