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THE ACTIXOMYCETES, Vol. II 



Glucose-asparagine agar: Growth hyaline 

 with pinkish reverse. Aerial mycelium pres- 

 ent. No soluble pigment. 



Calcium malate agar: Growth poor. No 

 aerial mycelium. 



Nutrient agar: Growth abundant, color- 

 less. Aerial mycelium whitish. Soluble pig- 

 ment amber. 



Starch: Strong hydrolysis. 



Potato: Growth abundant. Aerial myce- 

 lium light gray. No soluble pigment. 



Gelatin: Partial liquefaction. No soluble 

 pigment. 



Milk: No coagulation; some peptoniza- 

 tion. 



Nitrate reduction: Negative. 



Cellulose: Good growth. 



Carbon utilization: Utilizes glucose, su- 

 crose, lactose, galactose, rhamnose, xylose, 

 inositol, sodium succinate, and others. Does 

 not utilize sucrose, rafrinose, glycine, or 

 sodium citrate. 



Antagonistic properties: Produces an anti- 

 bacterial agent, matamycin. 



Habitat: Soil. 



Remarks: Related to S. noboritoensis and 

 S. spiralis. 



Characteristic properties: Culture said to 

 be melanin-positive; it produces gray to 

 violet spore masses. 



151. Streptomyces mediocidicus Okami et 

 al, 1954 (Okami, Y., Utahara, R., Naka- 

 mura, S., and Pmezawa, H. J. Antibiotics 

 (Japan) 7 A: 98-103, 1954). 



Morphology: Aerial mycelium sometimes 

 produces verticils, depending on composition 

 of medium; no spirals. 



Glycerol nitrate agar: Growth colorless to 

 yellowish. Aerial mycelium absent, or white 

 patches. Soluble pigment absent, or slightly 

 yellowish brown. 



Glucose-asparagine agar: Same as above. 



Starch agar: Same as above. Diastatic 

 action weak or medium. 



Nutrient agar: Growth colorless or slightly 



yellowish. No aerial mycelium. Soluble pig- 

 ment brownish. Melanin-positive. 



Potato: Growth yellowish or light yellow- 

 ish-brown. Aerial mycelium absent or white. 

 No soluble pigment. 



Gelatin: Growth yellowish-brown. No 

 aerial mycelium. Soluble pigment brown. 

 Strong liquefaction. 



Blood agar: Growth yellowish-brown to 

 reddish-brown. Aerial mycelium absent or 

 white. Hemolytic action strong. 



Milk: Surface ring colorless to yellowish. 

 No aerial mycelium. Soluble pigment 

 slightly brownish. Slow coagulation and 

 peptonization. 



Nitrate: No reduction. 



Antagonistic properties: Produces an anti- 

 fungal substance, mediocidin, a polyene of 

 the hexaene type. 



Type culture: IMRU 3777. 



152. Streptomyces melanocyclus (Merker, 

 1911, emend. Krainsky) Waksman and 

 Henrici, 1948 (Merker, E. Centr. Bakteriol. 

 Parasitenk. Abt, II, 31: 589, 1912; Krainsky, 

 A. ibid. 41:649-688, 1914). 



Morphology: Spores spherical, 0.9 /x. 



Agar media: Growth much folded, red. 

 Aerial mycelium dark brown. Soluble pig- 

 ment dark brown, turns whole culture red- 

 brown to almost black with a shade of red. 



Calcium malate agar: Colonies small, flat, 

 orange-red. Aerial mycelium black, occur- 

 ring along the edges. 



Gelatin: Growth poor. Liquefaction rapid. 



Milk: Coagulation and rapid peptoniza- 

 tion. 



Starch: Hydrolysis. 



Cellulose: Good decomposition; black cir- 

 cles produced on paper. 



Nitrate reduction: Positive. 



Sucrose: Inverted. 



Pigment: Insoluble in water and in or- 

 ganic solvents. Considered by Kriss to be 

 related to the melanins. 



Antagonistic properties: Strong effect 



