244 



THE ACTINOMYCKTES, Vol. II 



Morphology: Spores spherical to rod- 

 shaped, often produced in pairs or in chains, 

 2.0 by 2 to 5 /x- 



Calcium malate agar: Colonics minute, 

 yellow. No aerial mycelium. 



Glucose-asparagine agar: Aerial myce- 

 lium produced late (12 days), rose-yellow. 



Nutrient agar: Colonies yellow. Aerial 

 mycelium produced late, yellowish-rose. 



Potato: Growth yellowish, slimy mass. 

 No aerial mycelium. Melanin-negative. 



Gelatin: Colonies small, yellowish. Lique- 

 faction rapid. 



Milk: Rapid coagulation and peptoniza- 

 tion. 



Invertase: Negative. 



Starch: Diastatic action strong. 



Cellulose: Growth scant, white. 



Nitrate reduction: Positive. 



Production of H 2 S: Negative. 



Antagonistic properties: Said to produce 

 a form of strep tothricin. 



Habitat: Soil. 



Remarks: According to Ettlinger et al. 

 (1958), this organism belongs to the S. 

 griseus series. 



Type culture: IMRU 3332; A TCC 13,231. 



156. Streptomyces mirabilis Ruschmann, 

 1952 (Ruschmann, G. Pharmazie 7: 542 

 550, 639-648, 823 -831, 1052). 



Morphology: Sporophores straight, with- 

 out spirals or curvature. 



Agar media: Aerial mycelium white, 

 cottony. 



Nutrient agar: Growth poor, forming 

 slimy surface. No aerial mycelium. 



Glucose agar: Growth grayish-brown. No 

 aerial mycelium. Soluble pigment brown. 



Potato: Growth good, lichenoid. Soluble 

 pigment dark brown to black. 



Gelatin: Good flaky growth. Rapid lique- 

 faction. Soluble pigment dark brown to 

 black. 



Milk: Surface growth covered with white, 

 fluffy aerial mycelium. Coagulation and 



peptonization positive. Liquefied portion 

 colored black. 



Fats: Ready utilization. 



Temperature: Optimum 2 ( .)°C. No growth 

 at 37°C. 



Antagonistic properties: Antagonistic ef- 

 fect strongest in freshly isolated cultures. 

 Property lost on cultivation; activity lost 

 first against gram-negative, rod-shaped bac- 

 teria, cocci remaining most sensitive. Pro- 

 duces antibiotic miramycin. 



Remarks: Highly proteolytic and lipoly- 

 tic. Grows best on complex organic media, 

 at slightly acid reaction, pH 6.0 to 6.6. 



157. Streptomyces mitakaensis Ami et al., 

 1958 (Arai, M., Karasawa, K., Nakamura, 

 S., Yonehara, H., and Umezawa, H. J. 

 Antibiotics (Japan) 11A: 14-20, 1958). 



Morphology: Sporophores short, 



branched; spirals produced. Spores spheri- 

 cal, 1.2 to 1.5 fx. 



Sucrose nitrate agar: Growth good, color- 

 less or white to dark yellowish-brown. Aerial 

 mycelium powdery, abundant, light gull- 

 gray. No soluble pigment. 



Glucose-asparagine agar: Growth good, 

 white to brown; reverse becomes dark 

 brown. Aerial mycelium powdery, abundant, 

 gray. No soluble pigment. 



Glycerol citrate agar: Growth good, 

 colorless or white to brownish-white; later 

 brownish-yellow. Aerial mycelium powdery, 

 abundant, whitish-gray in center, gray in 

 the edges. No soluble pigment. 



Nutrient agar: Growth good, colorless to 

 pale yellowish-brown. Aerial mycelium pow- 

 dery, abundant, white, with or without 

 gray parts. No soluble pigment. 



Starch agar: Growth good, colorless to 

 pale yellowish-brown. Aerial mycelium pow- 

 dery, abundant, light gray in center, gray 

 at the edges. No soluble pigment. Hydroly- 

 sis strong. 



Potato plug: Growth good, wrinkled, 

 cream-buff. Aerial mycelium poorly devel- 



