250 



THE ACTINOMYCETES, Vol. II 



Antagonistic properties: Produces anti- 

 biotic homom y cin-hygromy cin . 



Remarks: S. noboritoensis belongs to the 

 group of chromogenic actinomycetes closely 

 related to S. cinnamonensis, S. fiavochromo- 

 genes, S. phaeochromogenes, S. aureus, and 

 S. tanashiensis. They differ in spiral forma- 

 tion, pigmentation on synthetic media, ni- 

 trate reduction, and production of anti- 

 biotics. 



169. Streptomyces nodosus Trejo, W. now 

 sp.* 



Morphology: Aerial mycelium forms open 

 and closed spirals, the latter predominating 

 as tightly knotted coils. Spores spherical to 

 oval, 0.5 to 1.0 by 1.0 /i. 



Sucrose nitrate agar: Growth white-green- 

 ish. Aerial mycelium pearl-gray to dawn- 

 gray. 



Nutrient agar: Substrate growth scant. 

 No aerial mycelium. No soluble pigment. 

 Melanin-negative. 



Oatmeal agar: Growth black with a buff 

 margin. Aerial mycelium deep olive-gray. 

 Reverse : olivaceus to black with a peripheral 

 ring of cream-buff to chamois. No soluble 

 pigment. 



Potato: Growth buff. Aerial mycelium 

 light olive-gray. No darkening of plug. 



Milk: Rapidly peptonized. 



Gelatin: Rapidly hydrolyzed. 



Nitrate reduction: Positive. 



Starch: Strong hydrolysis. 



Tyrosine: Utilized with no melanin forma- 

 tion. 



Carbon utilization: Utilizes mannitol, in- 

 ositol, rhamnose, xylose, D-fructose, treha- 

 lose, and melibiose. Docs not utilize adoni- 

 tol, sorbitol, arabinose, cellulose, sucrose, 

 lactose, sodium acetate, esculin, or dextrin. 



Antagonistic properties: Produces an anti- 

 fungal antibiotic, amphotericin. 



* Personal communication from Squibb Insti 

 tute for Medical Research (1958). 



Source: Isolated from soil in South 

 America. 



Remarks: This culture was specially de- 

 scribed for this treatise. It appears to be 

 closely related to S. rutgersensis. 



170. Streptomyces noursei Hazen and 

 Brown, 1950 (Hazen, E. L. and Brown, R. 

 Science 112: 423, 1950; Proc. Soc. Exptl. 

 Biol. Med 76: 93, 1951; Science 117: 609, 

 1953). 



Morphology: Sporophores produced as 

 side branches of sterile aerial hyphae; occa- 

 sionally produce open spirals and, according 

 to Ettlinger et al. (1958), also some verticils. 

 Spores round to oval, with thin long spines 

 (PL Ie). 



Sucrose nitrate agar: Growth scanty, col- 

 orless, flat. No aerial mycelium. 



Glucose-asparagine agar: Growth wrin- 

 kled, tan-colored, with gray and white knob- 

 like projections. Reverse of growth dark 

 gray. Aerial mycelium white, then reddish- 

 gray, finally ash-gray; limited shell-pink 

 diffusible pigment. 



Glucose-peptone agar: Growth good, 

 folded, brown. Aerial mycelium white, turn- 

 ing gray. Soluble pigment brown or pome- 

 granate-purple. 



Starch agar: Growth in form of discrete 

 colonies. Aerial mycelium white in center, 

 periphery colorless and embedded. Hydroly- 

 sis. 



Potato: Growth folded. Aerial mycelium 

 chalky white. At 35-36°C a reddish-purple 

 pigment is formed. 



Gelatin: Rapid liquet' action. Melanin- 

 negative. 



Milk: Coagulation, followed by peptoni- 

 zation. 



Cellulose: Growth poor. 



Nitrate: Traces of nitrite produced. 



Production of H..S: Negative. 



Blood agar: Growth consists of convex, 

 lobate colonies, with central perforation. 

 Aerial mycelium heavy, chalky white. No 

 hemolysis, but darkening of blood. 



