DESCRIPTION OF SPECIES OF STREPTOMYCES 



253 



Sucrose nitrate agar: Growth while, 

 spreading. Aerial mycelium ash-gray with 

 brownish tinge. No soluble pigment. 



Glycerol malate agar: Growth colorless. 

 Aerial mycelium light grayish-olive to dark 

 gray. 



Glucose-asparagine agar: Growth abun- 

 dant, natal-brown to almost black. Aerial 

 mycelium white with gray tinge. Soluble 

 pigment brownish. 



Nutrient agar: Growth wrinkled, brown, 

 becoming gray-green. Aerial mycelium 

 white. Soluble pigment brown. 



Starch agar: Growth transparent, spread- 

 ing. Aerial mycelium buff-gray. Rapid hy- 

 drolysis. 



Potato: Colonies small, wrinkled, black. 

 Xo aerial mycelium. Soluble pigment black. 



Gelatin: Surface growth cream-colored, 

 spreading. Aerial mycelium white. Soluble 

 pigment dark brown to deep olive-green. 

 Slow liquefaction. 



Milk: Dark brown ring. Coagulation and 

 peptonization. 



Cellulose: Growth faint. 



Nitrate reduction: Faint reduction to ni- 

 trite. 



Sucrose: [nvertase positive with good 

 growth. 



Temperature: Optimum, 37°C. 



Antagonistic properties: Positive. 



Habitat: Soil, water, river mud. 



Remarks: Ettlinger et al. (1958) consid- 

 ered this organism as a strain of S. griseus. 

 Krassilnikov (1949) considered it as a vari- 

 ety of .1 . chromogenes. 



176. Streptomyces olivoreticuli Arai et "/., 

 1957 (Arai, T., Nakada, T., and Suzuki, 

 M. Antibiotics & Chemotherapy 7: 435-442, 

 1957). 



Morphology: Sporophores form primary 

 and secondary verticils; secondary may also 

 be formed as tip clusters. Spores spherical 

 to oval. 



Sucrose nitrate agar: Growth thin, yellow 

 to brown. Aerial mycelium scant, later be- 



coming cottony, white with yellowish tinge. 

 Soluble pigment faint brown or absent . 



Glucose-asparagine agar: Growth thin, 

 light brown to olive-drab. Aerial mycelium 

 cottony, white with faint yellow to grayish- 

 pink t inge. 



Nutrient agar: Growth limited, brownish. 

 Aerial mycelium grayish- white. Soluble pig- 

 menl light blown. 



Gelatin: Surface growth poor. Liquefac- 

 tion slow, later becoming rapid. Soluble pig- 

 ment brown. 



Blood agar: Strong hemolysis. 



Potato: Growth wrinkled, dark brown. 

 Aerial mycelium abundant, powdery, cream- 

 colored to tea-green. Soluble pigment brown. 



Milk: King on surface brown. Coagulation 

 with limited peptonization. Soluble pigment 

 brown. 



Nitrate reduction: Negative. 



Si arch: Hydrolysis. 



Cellulose: No decomposition. 



Antagonistic properties: Produces anti- 

 biotic viomycin. 



177. Streptomyces olivoverticillatus Shi- 

 nobu, 1956 (Shinobu, R. Mem. Osaka Univ. 

 B (N. S.) 5: 84-93, 1956). 



Morphology: Sucrose-ammonium agar 

 mosl suitable for microscopic study. Pri- 

 mary and secondary verticils produced, 

 branches issuing sometimes closely, near the 

 top of the sporulating hyphae, forming 

 cluster-like or tuft-like branches. Spores 

 spherical to elliptical, 0.6 to 0.8 n. 



Sucrose nil rate agar: Trace of growth. 



Glucose-asparagine agar: Growth thin, 

 modeiate, pale olive to pale dark yellow. 

 Aerial mycelium thin, partially yellowish- 

 gray. 



Nutrient agar: Growth heavy, deep 

 brown. Aerial mycelium olive-gray to yellow 

 to green. Soluble pigment brown. 



Potato: Growth heavy, brown. Aerial 

 mycelium yellow-white to yellow-gray. Solu- 

 ble pigment brown. 



