156 



THE ACTINOMYCETES, Vol. II 



sidered S. parvus as belonging to the S. 

 griseus series. According to Gause et al. 

 (1957), S. parvus is a member of the scries 

 Fradiae. 



Type culture: IMRU 3686. 



183. Streptomyces pelletieri ( Laveran, 1906) 

 nov. comb. (Laveran, S. Compt. rend. soc. 

 biol. 61: 340, 1906). 



Morphology: Growth red, smooth, con- 

 sisting of small, dense, pink colonics. Myce- 

 lium nonsegmented, branched; hyphae 

 slender, straight, and not very long. Aerial 

 hyphae few, straight. 



Glucose-asparagine agar: Growth in form 

 of small, hard, red or purple adherent col- 

 onics. Xo soluble pigment. 



Glucose agar: Growth poor, in form of 

 minute, pink colonies. 



Glycerol agar: Growth poor, as few moist, 

 pink colonies. 



Nutrient agar: Colonies minute, colorless, 

 piled up into pale pink masses. 



Potato: Growth sparse, yellowish-pink, 

 irregularly piled up; later, abundant, small, 

 rounded, pink masses. Aerial mycelium 

 scant, white. 



Blood agar: Colonies at first a few pin- 

 head, cream-colored; no hemolysis. Later, 

 colonies are dense, button-shaped, with 

 narrow, fringed margin. 



Dorset's egg medium: Growth abundant, 

 wrinkled, pink skin with small discrete 

 colonies at margin; later, surface rough, 

 mealy, with considerable liquefaction. 



Gelatin: Few pink flakes. At first slow, 

 later almost complete liquefaction. 



Milk: Soft curd; gradual peptonization. 



Starch: Xo hydrolysis. 



Production of H 2 S: Negative. 



Source: Mycetoma in Nigeria. 



Remarks: In the original description of 

 tlii- culture by Laveran, the organism was 

 called Micrococcus pelletieri, because no my- 

 celium was seen, only coccoid bodies. A'. 

 indica was regarded as identical by Pinoy. 

 A', genesii Froes was described as closely 



allied (Erikson, 1935); the distinction was 

 founded upon the fact that the red grains 

 were smaller and much more numerous. A. 

 africanus is considered as a synonym of this 

 organism. According to Mariat (1958), S. 

 pelletieri hydrolyzes gelatin, serum albumin, 

 casein, and egg albumin; it utilizes urea but 

 not (XH 4 ) 2 S04 and KN0 3 as nitrogen 

 sources; it does not utilize xylose, galactose, 

 maltose, starch, mannitol, or paraffin as car- 

 bon sources. The species S. africanus is 

 indistinguishable from S. pelletieri. 



184. Streptomyces pentaticus Umezawa 

 and Tanaka, 1958 (Umezawa, S. and 

 Tanaka, Y. J. Antibiotics (Japan) 11A: 26- 

 29, 1958). 



Morphology: Straight sporophores pro- 

 duce primary and secondary verticils. Spores 

 can scarcely be observed. 



Sucrose nitrate agar: Growth poor, trans- 

 parent, penetrates deeply into medium. No 

 aerial mycelium. No soluble pigment. 



Glucose-asparagine agar: Growth color- 

 less, becoming purplish-pink to dull red- 

 purple, deep into medium. Aerial mycelium 

 white, sometimes pink. Soluble pigment 

 faint brown. 



Calcium malate agar: Growth red, irreg- 

 ular margin. No aerial mycelium. Soluble 

 pigment faint brown. 



Nutrient agar: Growth wet, colorless or 

 brownish-white. Xo aerial mycelium. Solu- 

 ble pigment brown. 



Starch agar: Growth colorless or pale 

 yellow, penetrates deeply into medium. Ae- 

 rial mycelium white, partially pinkish cot- 

 tony colonies. No soluble pigment. Starch 

 hydrolyzed. 



Gelatin: Growth consists of reddish colo- 

 nies produced on surface. Xo aerial myce- 

 lium. Soluble pigment deep brown. Rapid 

 liquefaction. 



Potato: Growth wrinkled, wet, grayish- 

 brown. Xo aerial mycelium. Soluble pig- 

 ment brownish-black. 



