DESCRIPTION OF SPECIES OF STREPTOM YCKS 



259 



Calcium malate agar: ( rrowth ochraceous- 

 salmon, becoming cinnamon-buff. Aerial 

 mycelium quaker-drab with areas <>l black 

 and while. Soluble pigmenl slight, greenish- 

 yellow. 



Nutrient agar: Poor substrate growth, 

 cream-yellow becoming buff to drab. Xo 

 aerial mycelium. Slight soluble brown pig- 

 ment. 



Starch agar: Growth cream- to buff-col- 

 ored. Aerial mycelium white, becoming 

 mouse-gray with patches of black. Slow 

 hydrolysis. 



Potato: Excellent growth. Aerial myce- 

 lium white to pale mouse-gray. Soluble pig- 

 ment brown. 



Gelatin: Very slow liquefaction. Melanin- 

 negative. 



Milk: Growth scant, forming partial ring 

 at surface. No coagulation or peptonization. 



Blood: Hemolysis. 



Cellulose: Growth slight. Aerial mycelium 

 gray to black. 



Nitrate: Reduction to nitrite, especially 

 with starch as source of carbon. 



Carbon utilization: Starch, malic acid, 

 inositol, sodium succinate, sodium citrate, 

 sorbitol, mannitol, maltose, arabinose, lac- 

 tose, galactose, fructose well utilized. Dul- 

 citol, raffinose, cellulose, sodium format* 1 , 

 sodium tartrate, xylose poorly utilized. 

 Asparagine, rhamnose, o-cresol, m-cresol, 

 sodium acetate, inulin, sodium salicylate 

 not utilized. 



Antagonistic properties: Produces oxytet- 

 racycline. 



Remarks: Tresner and Backus (1956) con- 

 sidered this organism as a variant of S. 

 hygroscopicus rather than a separate species. 

 Ettlinger et al. (1958) came to similar con- 

 clusions. 



190. Streptomyces pluricolor (Berestnew, 

 1897 emend. Krassilnikov, 1941) Waksman 

 (Krassilnikov, N. A. Actinomycetales. 

 Izvest. Akad. Xauk. SSSR, Moskau, p. 17, 

 1949). 



Morphology: Sporophores produce nu- 

 merous spirals, with .'5 to 5 turns (sinistrorse). 

 Spores oval, 0.9 by 0.7 y.. 



Synthetic agar: ( rrowth at first pigmented 

 yellow-red, Inter becoming bine to blue- 

 green. Aerial mycelium white-gray. The blue 

 pigment dissolves into the medium. 



Nutrient agar: Soluble pigmenl greenish, 

 fluorescent. 



Potato: Growth and soluble pigment 

 sharp blue. 



Gelatin: Liquefaction rapid. 



Milk: Peptonization positive; no coagula- 

 tion. 



Starch: Hydrolysis. 



Cellulose: No growth. 



Nutrient broth: Soluble pigment green, 

 fluorescent . 



Sucrose: Inversion. 



Antagonistic properties: None. 



Habitat: Soil. 



Remarks: Closely related to S. violaee- 

 oruber. A. pluricolor diffundens Berestnew is 

 considered by Krassilnikov as a synonym. 



191. Streptomyces phuicolorescens Okami, 

 Y. and Umezawa, H.* n. sp. 



Morphology: Aerial hyphae not flexuous, 

 few branches; no spirals. 



Glycerol nitrate agar: Growth at first 

 colorless and yellowish, then yellowish- 

 brown with reddish tone. Aerial mycelium 

 white to olive or pinkish. Soluble pigment 

 slightly yellowish-brown or light wine-color 

 with aging. 



Glucose-asparagine agar: Growth at first 

 colorless and yellowish, then yellowish- 

 brown with reddish tone. Aerial mycelium 

 white to olive or pinkish. Soluble reddish- 

 purple pigment occasionally produced. 



Calcium malate agar: Same as on glycerol 

 nitrate agar. 



Nutrient agar: :>7°C. Colorless or slight 

 yellowish-brown growth with fine wrinkles. 

 Aerial mycelium white. Soluble pigmenl ab- 

 sent at first, later a brown pigment appears. 



* Personal communication from Okami. 



