2(14 



THE ACTINOMYCETES, Vol. II 



A I nephology : Sporophores spiral-shaped. 

 Spores spherical. 



Nutrient agar: Growth colorless to gray- 

 ish. Aerial mycelium white. No soluble pig- 

 ment. 



Potato: Growth folded, sulfur-yellow. 

 Aerial mycelium chalk- white. No soluble 

 pigment . Melanin-negative. 



Milk: Pellicle heavy. Aerial mycelium 

 white. Peptonization gradual without pre- 

 vious coagulation. 



Loefrler's serum: Growth yellow. No aerial 

 mycelium. Serum liquefied and colored yel- 

 lowish-brown. 



Odor: Strong, putrefactive. 



Habitat: Spring water. 



Remarks: Decomposes proteins ener- 

 getically, with the formation of bad-smelling 

 products (H 2 S, NH 3 ). Morphological prop- 

 erties given by Krassilnikov (1949), who 

 considers this organism as a member of the 

 .4. albus group. 



202. Streptomyces pyridomyceticus Okami 

 et a/., 1957 (Okami, Y., Maeda, K., and 

 Umezawa, H. J. Antibiotics (Japan) 7A: 

 55-50, 1954; 10A: 172, 1957). 



Morphology: Sporophores form flexible, 

 open spirals. Spores of irregular size. 



Glycerol nitrate agar: Growth colorless 

 to dark. Aerial mycelium thin, white, some- 

 times gray to brownish-gray. No soluble 

 pigment. 



Nutrient agar: Growth colorless. Aerial 

 mycelium absent, or scant, white. No soluble 

 pigment. 



Potato: Growth wrinkled, dark yellowish- 

 brown. Aerial mycelium absent, or later 

 while. No soluble pigment. 



Gelatin: Growth colorless. Aerial myce- 

 lium white, sometimes grayish. No soluble 

 pigment. No liquefaction. 



Starch: Hydrolysis. 



Milk: Growth yellowish, in the form of 

 surface ring. No aerial mycelium. Coagula- 

 tion and peptonization absent or very slow. 



Blood: No hemolysis. 



Nitrate reduction: Negative. 



Carbon utilization: Utilizes arabinose, 

 dextrin, fructose, galactose, glucose, glycerol, 

 maltose, xylose, and sucrose. Does not utilize 

 dulcitol, esculin, inulin, lactose, mannose, 

 raffinose, rhamnose, salicin, sorbitol, sodium 

 citrate, sodium acetate, and sodium suc- 

 cinate. 



Habitat: Soil in Japan. 



Antagonistic properties: Produces anti- 

 biotic pyridomycin. 



Remarks: Isolated by means of chlortetra- 

 cycline-containing agar. Related to S. cacaoi 

 and S. flocculus, as well as to S. hygroscopicus. 

 Above description was first given under the 

 name S. olbidofuscus. It was later found that 

 this name was preempted by Neukirch and 

 Berestnew, and was, therefore, changed to 

 S . pyridom ycet ic us. 



203. Streptomyces rameus Okami et al., 

 1959) (Okami, Y., Suzuki, M., Takita, T., 

 Ohi, K., and Umezawa, H. J. Antibiotics 

 (Japan) 12A: 257-202, 1959). 



Morphology: Aerial mycelium forms in- 

 complete spirals or loops or hooks. Spores 

 oval to oblong. 



Glycerol nitrate agar: Growth yellow. 

 Aerial mycelium white. Soluble pigment 

 absent or yellowish. 



Glucose-asparagine agar: Growth yellow- 

 ish. Aerial mycelium white. Soluble pig- 

 ment absent or yellowish. 



Calcium malate agar: Growth colorless to 

 yellowish. Aerial mycelium off-white. No 

 soluble pigment. 



Nutrient agar: Growth colorless to brown- 

 ish. Aerial mycelium white. Soluble pigment 

 absent or slight brownish. 



Starch agar: Growth colorless to yellowish. 

 Aerial mycelium white. Soluble pigment 

 yellowish. Hydrolysis weak to medium. 



Potato: Growth brownish. Aerial myce- 

 lium white. Soluble pigment brown to black. 



Gelatin: Growth brownish. Aerial myce- 

 lium white. Soluble pigment brown. Liquefac- 

 tion doubtful. 



