DESCRIPTION OF SPECIES OF STREPTOMYCBS 



265 



Milk: Coagulation weak; peptonization 

 doubtful. 



Nitrate reduction: Negative. 



Carbon sources: Utilizes arabinose, dex- 

 trin, fructose, galactose, glucose, glycerol, 

 inositol, maltose, niannitol, mannose, raffi- 

 nose, starch, and sucrose. Lactose and xylose 

 gave less response. Poor growth on inulin, 

 rhamnose, salicin, sorbitol, sorbose, sodium 

 acetate, and sodium citrate. 



Antagonistic properties: Produces strepto- 

 mycin. 



Remarks: belated to S. alboflavus, S. 

 xanthophaens, and S. orientalis. 



204. Streptomyces ramnaii Bhuiyan and 

 Ahmad, L956 (Bhuiyan, A. M. and Ahmad, 

 K. Ann. Biochem. Exptl. Med. India 16: 

 101-104, 1956). 



Morphology: Open spirals, with 2 or ."> 

 turns. Spores spherical, 0.S /i in diameter. 



Sucrose nitrate agar: Growth whitish. 

 Aerial mycelium white to pale rose. No 

 soluble pigment. 



Glucose-asparagine agar: Growth color- 

 less to pale rose. Aerial mycelium white, 

 later pale rose. 



Calcium malate agar: Growth smooth, 

 cream-colored. Aerial mycelium white. Me- 

 dium becomes clear. 



Nutrient agar: Growth cream-colored, be- 

 coming light brown. Aerial mycelium pow- 

 dery, white. Soluble pigment slight blown 

 coloration. Melanin-negative. 



Starch agar: Growth cream-colored to 

 yellowish brown. No aerial mycelium. Xo 

 soluble pigment. Hydrolysis rapid. 



Potato: Growth abundant, cream-colored. 

 Aerial mycelium white, turning pale rose. 



Potato nutrient agar: Growth rapid, color- 

 less to cream-colored. Aerial mycelium deep 

 rose. Soluble pigment deep reddish-brown 

 to almost red. 



Gelatin: Growth cream-colored. Xo aerial 

 mycelium. Soluble pigment absent or light 

 brown. Liquefaction medium. 



.Milk: Growth cream-colored. Xo aerial 



mycelium. Coagulation, followed by pep- 

 tonization. Read ion acid. 



Nitrate reduction: Positive. 



Cellulose: ( irowth good. 



< )ptimum temperature: 37°C. 



Antagonistic properties: Produces anti- 

 biotic ramnacin. 



205. Streptomyces ramulosus Ettlinger <t 

 til., 1958 (Ettlinger, L., Gaumann, P., Flut- 

 ter, P., Keller-Schierlein, W., Kradolfer, 

 P., Xeipp, P., Prelog, V., and Zahner, H. 

 Ilelv. Chun. Acta 41: 216-219, 1958). 



Morphology: Sporophores monopodia I ly 

 branched, straight with many side branches. 

 Spores smooth (PI. II h ). 



Glycerol nitrate agar: Growl h al first 

 carmine-red, later turning greenish-brown. 

 Aerial mycelium ash-gray with greenish 

 tinge. Substrate pigmented carmine-red. 



Glucose-asparagine agar: Growth yellow- 

 ish-red. Aerial mycelium gray. Substrate 

 carmine-red. 



Calcium malate agar: ( irowth light yellow. 

 Aerial mycelium chalky white to gray with 

 greenish tinge. 



Glucose-peptone agar: Growth yellowish- 

 red, partly greenish to greenish-black. Aerial 

 mycelium powdery, ash-gray. Substrate 

 greenish to brownish-black. 



Starch agar: Growth light yellow. Sub- 

 strate light carmine. Gradual hydrolysis. 



Potato: Growth yellowish-red. Aerial my- 

 celium chalk-white to ash-gray. Substrate 

 carmine-red. 



Gelatin: Growth light yellow. Soluble pig- 

 ment light brown. Xo liquefaction. 



Milk: Light yellow pellicle. Xo aerial 

 mycelium. Coagulation limited; peptoniza- 

 tion good. 



Carhon utilization: Glucose, L-xylose, d- 

 fructose, sucrose, inulin, D-sorbitol well 

 utilized. Does not utilize raflinose, L-arabi- 

 nose, D-mannitol, mesoinositol. Question- 

 able utilization of L-rhamnose, salicin. 



Antagonistic properties: Produces anti- 

 biotic acetomycin, active against gram-posi- 



