DKSCHII'TIOX OF SPKCIICS ol' STItKI'TOUYCES 



207 



be identical with S. abikoensum. Sakagami 

 et al. (1958) described a variety latumcidicus 

 thai produced no aerial mycelium on mosl 



media, and formed the antibiotic latumcidin. 

 Type culture: IMRU 3344. 



208. Streptomyces rimosus Sobin et al., 

 1950 (Sobin, B. A., Finlay, A. ('., and Kane, 

 J. H. U. S. 2,510,080, July IS, 1050; see also 

 Koehi, M., et al. Proc. Natl. Acad. Sci. U. S. 

 38:883-891, 1952). 



Morphology: Sporophores long, usually 

 straight, occasionally open or closed spirals 

 depending on composition of medium. Spores 

 cylindrical, 0.6 to 0.7 by 0.8 to 1.4 M - A 

 microscopic study of S. rimosus (strain 3558 i 

 grown on yeast extract-glucose agar, after 

 14 days incubation, revealed the following: 

 Aerial hyphae were long and fairly straight, 

 segmenting into chains of even, bead-like 

 -poics. Other aerial hyphae were long, 

 tangled, branching, twisting into spirals, 

 also segmented into chains of bead-like 

 spores. 



Sucrose nitrate agar: Growth thin, cream- 

 colored, developing slowly at first, later be- 

 coming abundant, much folded or lichenoid; 

 reddish-brown to orange. Aerial mycelium 

 appears first over the drier edge of the 

 growth or in the form of thin white patches. 

 When the culture becomes older, a faint 

 bluish zone appears around the edge of the 

 growth. Soluble pigment faint yellowish. 



Glucose-asparagine agar: Growth at first 

 cream-colored, becoming brownish to orange- 

 brown with age. Aerial mycelium white. 

 Soluble pigment yellowish to golden. 



Yeast-glucose agar: Growth much more 

 rapid than in synthetic media; lichenoid, 

 cream to brownish. Aerial mycelium appears 

 at an early stage of growth, white, later 

 tending to become mouse-gray. Soluble pig- 

 ment yellowish. 



Nutrient agar: Growth poor, cream- 

 colored to yellowish-brown to mouse-gray. 

 Aerial mycelium white or absent. Soluble 

 pigment absent or yellowish. 



Starch agar: Growth limited, cream- 

 colored, with deeper brown center. No aerial 

 mycelium. Limited hydrolysis. 



Potato: Growth lichenoid, cream-colored 

 t<> reddish-brown. Aerial mycelium white to 

 gray to dark brown. Soluble pigmenl yel- 

 lowish-brown. 



Gelatin: Growth cream-colored to brown- 

 ish. Aerial mycelium white. Slow liquefac- 

 tion. No soluble pigment, only a faint yel- 

 lowish coloration of liquefied portion. 



Milk: Heavy surface pellicle, cream- 

 colored to yellowish. Aerial mycelium gray- 

 ish-white. Peptonization, without coagula- 

 tion. 



Cellulose: Xo growth. 



Nitrate reduction: Positive. 



Production of H 2 S: Negative. 



Antagonistic properties: Produces an anti- 

 bacterial antibiotic, oxy tetracycline, and an 

 antifungal agent, rimocidin. 



Habitat : Soil. 



Remarks: A variety of S. rimosus (forma 

 paromomycinus) was briefly described (Brit. 

 Pat. 797,568, July 2, 1958). This variety was 

 isolated from a soil in South America. It dif- 

 fers from S. rimosus in certain minor cultural 

 properties (somewhat lighter color on agar 

 media) and in poorer utilization of arabinose. 

 Both form dense clusters of spirals on various 

 synthetic media and on glucose-tryptone 

 agar. The variety produces an antibiotic, 

 paromomycin, apparently closely related to 

 the neomycin group. 



Type culture: IMRU 3558; ATCC 1(),<>7() 



209. Streptomyces rochei Berger et al., 

 L949 (Berger, J., Jampolsky, L. Al., and 

 Goldberg, M. W. Arch. Biochem. 22: 476- 

 478, 1949; Waksman, S. A. and Lechevalier, 

 H. Guide to the classification and identifica- 

 tion of the actinomycetes and their anti- 

 biotics. The Williams and Wilkins Co., 

 Baltimore, 1953, p. 40). 



Morphology: Sporophores straight, 1.5 ^ 

 in diameter; often, but no1 always spirally 

 twisted; spirals usually shorl and loose with 



